Detailed information of TA system TA048752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 4454..5945
• Replicon: plasmid pLA5
• Accession: NZ_CP009295
• Organism: Novosphingobium pentaromativorans US6-1

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: G6EKC7
• Locus tag: JI59_RS24475
• Protein ID: WP_007015705.1
• Coordinates: 4454..5719 (-)
• Length: 422 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: JI59_RS24480
• Protein ID: WP_007015704.1
• Coordinates: 5709..5945 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI59_RS24460	1..1194	+	1194	WP_007015708.1	AAA family ATPase	-
JI59_RS24465	1304..2377	+	1074	WP_007015707.1	ParB/RepB/Spo0J family partition protein	-
JI59_RS24470	2484..3761	+	1278	WP_007015706.1	replication initiator protein A	-
JI59_RS24475	4454..5719	-	1266	WP_007015705.1	type II toxin-antitoxin system HipA family toxin	Toxin
JI59_RS24480	5709..5945	-	237	WP_007015704.1	helix-turn-helix transcriptional regulator	Antitoxin
JI59_RS24485	6277..6756	+	480	WP_007015703.1	hypothetical protein	-
JI59_RS24490	6774..7385	+	612	WP_138921566.1	hypothetical protein	-
JI59_RS24495	7382..7870	+	489	WP_007015701.1	hypothetical protein	-
JI59_RS24500	7964..8482	+	519	WP_007015700.1	DUF2384 domain-containing protein	-
JI59_RS24505	8790..9827	+	1038	WP_007015769.1	tyrosine-type recombinase/integrase	-
JI59_RS24510	10064..10552	+	489	WP_007015770.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..134722	134722

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (7-64)

Sequences

Toxin

Protein

• Length: 422 a.a.
• Molecular weight: 44252.73 Da
• Isoelectric Point: 5.7734

>T48752 WP_007015705.1 NZ_CP009295:c5719-4454 [Novosphingobium pentaromativorans US6-1]
MAADTLDRLVVWSNRRIAGDLVIDLGGAMHFAYAEDWLADRGAPALSHALPKQAGAFGDALCKAVFGGLLPEEGQRTAIA
RALGVSPDNPFLLLAALGGDVAGDLAFLPPGEQPPPAPTERPAEALDDAALADLLQRLPRVPMLAGEGGARLSLAGAQGK
LPVVLANGRIAVPRIGEPSTHLIKPEPERFPGLAANEAFCLALARAAGLDAATGQWQAVAGKPYLLVARYDRVERNGQIQ
RLHQEDFAQALGVPSNRKYASEGGPAFRDCFALVRQATTRPAREVLKLADAAIFNLIIGNADAHAKNFSLLRREDGEVVL
APLYDLVATFIWPELSARLAMRFGPAASLEEVDGDSLIGFAQDSGLTLPFLRRRAAALAGQVEAAIARGIEVPGLAATAL
PGELSQAISDRAGRLALKARG

Nucleotide

• Length: 1266 bp

>T48752 NZ_CP009295:c5719-4454 [Novosphingobium pentaromativorans US6-1]
ATGGCTGCTGACACTCTCGACCGCCTCGTCGTCTGGTCCAATCGCCGCATCGCGGGCGACCTTGTGATCGACCTCGGCGG
GGCGATGCATTTCGCCTATGCCGAGGACTGGCTGGCGGATCGCGGGGCCCCTGCCCTGTCCCACGCCCTGCCCAAGCAGG
CGGGAGCCTTCGGCGATGCCTTGTGCAAGGCCGTGTTCGGCGGGCTGCTTCCCGAGGAAGGGCAGCGCACGGCGATTGCC
CGTGCGCTGGGCGTTTCACCCGACAACCCGTTCCTGCTGCTGGCGGCGCTGGGCGGCGATGTTGCAGGCGACCTTGCCTT
CCTGCCCCCGGGGGAGCAACCGCCTCCGGCCCCGACCGAAAGGCCCGCAGAGGCGCTCGATGACGCGGCGCTCGCCGATC
TTCTCCAGCGCCTGCCCCGGGTTCCCATGCTGGCGGGCGAAGGGGGGGCACGCCTCAGCCTTGCCGGGGCGCAGGGCAAG
CTCCCCGTGGTCCTGGCAAACGGGAGGATCGCCGTACCGCGCATCGGCGAGCCTTCGACCCACCTGATCAAGCCGGAGCC
CGAACGCTTTCCCGGTCTGGCCGCCAATGAGGCCTTCTGTCTTGCGCTTGCGCGCGCTGCCGGGCTCGATGCAGCGACCG
GGCAGTGGCAGGCAGTTGCCGGGAAGCCCTACCTGCTCGTCGCGCGCTACGACCGGGTTGAAAGGAACGGGCAGATCCAG
CGGCTGCATCAGGAAGATTTCGCGCAGGCGCTGGGCGTGCCGTCCAACCGGAAATACGCCAGCGAGGGCGGACCCGCCTT
CCGCGATTGCTTTGCGCTCGTGCGGCAGGCCACCACGCGCCCGGCGCGGGAGGTTCTCAAGCTGGCCGATGCAGCGATCT
TCAACCTGATCATCGGCAATGCCGACGCCCATGCCAAGAATTTCAGCCTGCTGCGCCGGGAGGATGGGGAAGTCGTCCTT
GCCCCGCTCTACGATCTGGTGGCGACGTTCATCTGGCCGGAACTTTCCGCAAGGCTCGCCATGCGCTTCGGCCCAGCTGC
CAGCCTCGAGGAAGTGGATGGCGACAGCCTGATAGGGTTCGCGCAGGACAGCGGCCTCACCCTGCCCTTCCTGCGCCGGC
GCGCCGCAGCTCTGGCTGGACAAGTCGAAGCGGCGATTGCCCGGGGTATCGAGGTGCCCGGTCTGGCAGCTACGGCACTC
CCCGGCGAACTCTCTCAGGCCATCTCGGACCGGGCGGGTCGACTGGCACTCAAGGCACGCGGATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8208.51 Da
• Isoelectric Point: 6.9491

>AT48752 WP_007015704.1 NZ_CP009295:c5945-5709 [Novosphingobium pentaromativorans US6-1]
MDTAGIGQIIREERKALGLHQDQLAAASGVGLRFIVELERGKVTAQIGKVIAVLSALGCEVQVLRPGDLRPGERGDGC

Nucleotide

• Length: 237 bp

>AT48752 NZ_CP009295:c5945-5709 [Novosphingobium pentaromativorans US6-1]
ATGGACACGGCCGGGATCGGGCAGATCATTCGCGAGGAACGCAAAGCGCTGGGCCTGCACCAAGATCAGCTGGCGGCAGC
AAGCGGGGTGGGCTTGCGTTTCATCGTCGAGTTGGAGCGAGGCAAGGTCACGGCGCAGATCGGCAAGGTGATTGCGGTCT
TGTCGGCGCTGGGCTGCGAAGTGCAGGTCCTGCGTCCCGGCGATCTCCGGCCGGGAGAGCGCGGCGATGGCTGCTGA

Structures

Toxin

• AlphaFold DB: G6EKC7