Detailed information of TA system TA048751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 124899..126349
• Replicon: plasmid pLA1
• Accession: NZ_CP009294
• Organism: Novosphingobium pentaromativorans US6-1

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: G6EKU5
• Locus tag: JI59_RS24145
• Protein ID: WP_007015873.1
• Coordinates: 124899..126140 (-)
• Length: 414 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: JI59_RS24150
• Protein ID: WP_007015874.1
• Coordinates: 126137..126349 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI59_RS24115	120719..121009	-	291	WP_007015867.1	ribbon-helix-helix protein, CopG family	-
JI59_RS24120	121484..121711	+	228	WP_007015868.1	hypothetical protein	-
JI59_RS24125	121839..122150	-	312	WP_007015869.1	hypothetical protein	-
JI59_RS27500	122147..122335	-	189	WP_007015870.1	hypothetical protein	-
JI59_RS24135	122617..123672	-	1056	WP_037486491.1	DUF2493 domain-containing protein	-
JI59_RS24140	124236..124517	+	282	WP_007015872.1	hypothetical protein	-
JI59_RS24145	124899..126140	-	1242	WP_007015873.1	type II toxin-antitoxin system HipA family toxin	Toxin
JI59_RS24150	126137..126349	-	213	WP_007015874.1	helix-turn-helix transcriptional regulator	Antitoxin
JI59_RS24155	126647..127162	-	516	WP_007015875.1	MucR family transcriptional regulator	-
JI59_RS24160	127281..127838	-	558	WP_007015877.1	hypothetical protein	-
JI59_RS24165	128339..128674	-	336	Protein_123	single-stranded DNA-binding protein	-
JI59_RS26510	129155..129498	-	344	Protein_124	thermonuclease family protein	-
JI59_RS24170	129812..130000	+	189	WP_138921579.1	hypothetical protein	-
JI59_RS24175	130018..130707	-	690	WP_007015881.1	pentapeptide repeat-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..188477	188477

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (147-378)

Antitoxin

• Cro/C1-type helix-turn-helix domain (7-61)

Sequences

Toxin

Protein

• Length: 414 a.a.
• Molecular weight: 45341.27 Da
• Isoelectric Point: 6.4311

>T48751 WP_007015873.1 NZ_CP009294:c126140-124899 [Novosphingobium pentaromativorans US6-1]
MSSRLTVWWDGRVVGALYLDPFGETQFAYDPGWLTELRAPALSFSLPKQVEPFHRRECQPFFGGILPEESQRTAIARALG
VSADNEFRLLEHLGGEVAGALTLLPEGEAPTPHSAAAPKPLDDDRLIQLLDHLPMRPMLAGEDGLRLSLAGAQSKLPVLL
IDRQVALPAPGQPTSHILKPPITRFPWTTENEYFCMSLARAIGLDVAPVEMRTVGDRSFLLISRYDRTIGPSGELVRLHQ
EDFAQALGVPSHRKYASEGGPNFPDCFALLRRAATRPPRDILRLLDAAIFNLIIGNADAHAKNFSLLHKDGAIELAPLYD
LLSTTLYPELSPKLAMKIGGKHLLNELEPRHWDKFAVDCGLGAPFVRTRIRQLCEAVIAGLDNEFTGPTWAADGFVPVIA
LVRERAGLLGGKL

Nucleotide

• Length: 1242 bp

>T48751 NZ_CP009294:c126140-124899 [Novosphingobium pentaromativorans US6-1]
ATGAGCAGCAGGCTCACCGTCTGGTGGGACGGCCGTGTGGTCGGCGCCCTCTATCTCGACCCCTTCGGCGAAACCCAGTT
TGCCTATGATCCAGGCTGGCTGACAGAGCTTCGCGCTCCTGCGCTTTCATTCTCGCTACCCAAGCAGGTCGAGCCATTCC
ATCGTCGGGAATGCCAGCCTTTCTTTGGCGGCATTCTGCCCGAGGAAAGCCAGCGTACTGCCATCGCCCGCGCACTTGGT
GTCTCCGCCGACAATGAATTCCGGCTGCTCGAGCATCTCGGCGGTGAAGTGGCAGGCGCTTTGACGCTCCTTCCTGAAGG
CGAAGCCCCCACTCCCCATAGCGCCGCAGCGCCCAAACCTCTCGATGATGATCGTCTGATCCAGTTGCTCGATCATTTGC
CGATGCGTCCAATGCTGGCGGGCGAGGATGGGTTGCGGCTGTCATTGGCCGGGGCACAATCGAAACTTCCGGTGCTTCTG
ATCGACAGGCAGGTTGCCCTGCCAGCACCCGGGCAGCCGACCAGTCATATCCTCAAACCACCGATCACGCGTTTCCCCTG
GACGACCGAGAACGAATACTTCTGCATGTCGCTCGCCCGCGCCATCGGCCTTGACGTTGCACCGGTCGAAATGCGTACGG
TTGGCGATCGTTCATTTCTGCTGATCAGTCGCTATGATCGGACCATCGGTCCGTCAGGCGAACTGGTCCGCCTGCATCAG
GAAGACTTCGCGCAGGCGCTTGGCGTGCCGTCACACCGCAAATATGCGAGCGAAGGCGGGCCGAATTTTCCTGATTGCTT
CGCGCTGCTGCGCCGGGCAGCAACCCGTCCGCCACGCGACATCCTGCGGCTGCTCGATGCGGCAATCTTCAACCTGATCA
TCGGCAATGCTGATGCCCACGCCAAGAATTTTAGCCTCCTGCACAAGGACGGCGCGATCGAGCTTGCACCGCTCTACGAT
CTGCTTTCGACCACGCTTTACCCGGAACTCTCGCCGAAGCTCGCGATGAAGATTGGCGGGAAGCACCTCCTCAACGAACT
CGAGCCGCGTCATTGGGACAAGTTCGCTGTCGATTGCGGGCTTGGCGCCCCGTTCGTGCGGACCCGGATCAGGCAATTGT
GTGAGGCTGTCATTGCCGGCCTGGACAACGAATTTACTGGCCCTACTTGGGCGGCAGATGGATTCGTCCCTGTCATCGCG
CTTGTCCGTGAGCGCGCCGGGCTGCTCGGTGGGAAGTTGTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7442.71 Da
• Isoelectric Point: 10.3137

>AT48751 WP_007015874.1 NZ_CP009294:c126349-126137 [Novosphingobium pentaromativorans US6-1]
MDSNSLGLLVRRERKAQNLKQAELAAVSGVGVRFIVDLEAGKPTLQLGKVLQVITTLGCEVQIAPPASRS

Nucleotide

• Length: 213 bp

>AT48751 NZ_CP009294:c126349-126137 [Novosphingobium pentaromativorans US6-1]
ATGGACAGCAATTCACTTGGCCTCCTTGTGCGCCGCGAACGCAAGGCACAGAACCTGAAGCAGGCAGAGCTTGCGGCAGT
GAGCGGCGTTGGCGTGCGGTTTATTGTCGATCTCGAAGCTGGCAAGCCGACGCTGCAACTCGGCAAGGTGCTTCAGGTCA
TCACGACGCTCGGCTGCGAAGTCCAGATCGCCCCGCCTGCGAGCCGGTCATGA

Structures

Toxin

• AlphaFold DB: G6EKU5