Detailed information of TA system TA048750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 262701..263371
• Replicon: plasmid pLA4
• Accession: NZ_CP009293
• Organism: Novosphingobium pentaromativorans US6-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JI59_RS23155
• Protein ID: WP_007015549.1
• Coordinates: 262701..263114 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G6EJX0
• Locus tag: JI59_RS23160
• Protein ID: WP_007015548.1
• Coordinates: 263111..263371 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI59_RS23115	258226..258447	-	222	WP_039858624.1	hypothetical protein	-
JI59_RS23120	258574..259176	-	603	WP_007015555.1	hypothetical protein	-
JI59_RS23125	259209..259538	-	330	WP_007015554.1	hypothetical protein	-
JI59_RS23130	259592..259921	-	330	WP_007015553.1	single-stranded DNA-binding protein	-
JI59_RS23135	260639..260938	+	300	WP_007015551.1	DUF1153 domain-containing protein	-
JI59_RS25850	261141..261467	+	327	WP_081474091.1	tyrosine-protein phosphatase	-
JI59_RS23150	261953..262495	-	543	WP_041565059.1	DUF736 domain-containing protein	-
JI59_RS23155	262701..263114	-	414	WP_007015549.1	type II toxin-antitoxin system VapC family toxin	Toxin
JI59_RS23160	263111..263371	-	261	WP_007015548.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JI59_RS23165	263457..264395	-	939	WP_007015547.1	hypothetical protein	-
JI59_RS23170	264455..265150	-	696	WP_007015546.1	hypothetical protein	-
JI59_RS23175	265308..265616	-	309	WP_007015545.1	hypothetical protein	-
JI59_RS23180	265621..266022	-	402	WP_007015544.1	hypothetical protein	-
JI59_RS23185	266025..266495	-	471	WP_007015543.1	hypothetical protein	-
JI59_RS23190	266498..267010	-	513	WP_007015542.1	hypothetical protein	-
JI59_RS26450	267157..268042	+	886	Protein_245	cation:proton antiporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..335704	335704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (15-52)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14610.82 Da
• Isoelectric Point: 4.8550

>T48750 WP_007015549.1 NZ_CP009293:c263114-262701 [Novosphingobium pentaromativorans US6-1]
VTNLYMLDTNIVSDLARNPQGAVAKRIADVGPDTICVSIITAAELRYGCAKKGSPRLLAQIEAILDSVQILALQVPADTE
YGGIRAELEAAGKPITPNDLFIAAHAYALEAVLVTANVGEFSRVRALKVENWLDGVH

Nucleotide

• Length: 414 bp

>T48750 NZ_CP009293:c263114-262701 [Novosphingobium pentaromativorans US6-1]
GTGACCAACCTCTACATGCTCGACACCAACATCGTGTCCGACCTTGCGCGCAATCCCCAGGGCGCCGTCGCGAAGCGGAT
CGCGGATGTCGGGCCGGACACGATCTGCGTCAGCATCATTACCGCCGCGGAACTGCGTTACGGTTGCGCGAAAAAGGGAT
CGCCGCGGTTGCTCGCTCAGATCGAAGCCATTCTCGACAGCGTGCAGATACTTGCGCTCCAAGTCCCCGCCGACACGGAA
TATGGCGGCATCCGCGCCGAACTCGAAGCCGCGGGCAAGCCCATCACCCCCAACGATCTCTTCATCGCTGCCCACGCCTA
TGCCCTCGAAGCGGTCCTAGTGACCGCCAATGTGGGTGAGTTCTCGCGGGTACGCGCGCTCAAGGTCGAGAACTGGCTGG
ACGGGGTCCATTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9645.10 Da
• Isoelectric Point: 4.8232

>AT48750 WP_007015548.1 NZ_CP009293:c263371-263111 [Novosphingobium pentaromativorans US6-1]
MAQSDLTPAREAKLFRNNKSQAVRIPADFEFPGDRVLIHREGDRLIIEPIRRKNLLEVLAGLEPLGAEDQFPDIDDSLLP
AKAIVL

Nucleotide

• Length: 261 bp

>AT48750 NZ_CP009293:c263371-263111 [Novosphingobium pentaromativorans US6-1]
ATGGCACAATCCGACCTCACCCCTGCTCGAGAGGCCAAGCTTTTCCGCAACAACAAAAGCCAGGCGGTGCGAATACCAGC
CGACTTCGAGTTTCCCGGCGACCGCGTTTTGATCCACCGCGAGGGCGACCGGCTCATTATCGAGCCAATCCGCCGCAAGA
ACTTGCTGGAAGTGCTGGCCGGACTGGAACCCCTTGGGGCGGAGGATCAGTTCCCCGATATCGATGATTCGCTGCTGCCA
GCCAAGGCCATCGTCCTGTGA

Structures

Antitoxin

• AlphaFold DB: G6EJX0