Detailed information of TA system TA048749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 389417..389967
• Replicon: plasmid pLA3
• Accession: NZ_CP009292
• Organism: Novosphingobium pentaromativorans US6-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G6EGT7
• Locus tag: JI59_RS26355
• Protein ID: WP_007014466.1
• Coordinates: 389665..389967 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: JI59_RS20475
• Protein ID: WP_039858067.1
• Coordinates: 389417..389662 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI59_RS20455	385103..386605	+	1503	WP_007014470.1	hypothetical protein	-
JI59_RS20460	386595..386984	-	390	WP_039858065.1	hypothetical protein	-
JI59_RS20465	387449..388273	+	825	WP_138921487.1	hypothetical protein	-
JI59_RS20470	388612..389244	-	633	WP_039858069.1	tyrosine-type recombinase/integrase	-
JI59_RS20475	389417..389662	+	246	WP_039858067.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
JI59_RS26355	389665..389967	+	303	WP_007014466.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JI59_RS20480	390386..390847	-	462	WP_007014465.1	hypothetical protein	-
JI59_RS20485	390853..392736	-	1884	WP_007014464.1	metallophosphoesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..756828	756828

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-93)

Antitoxin

• Antitoxin ParD (1-74)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11270.64 Da
• Isoelectric Point: 5.4710

>T48749 WP_007014466.1 NZ_CP009292:389665-389967 [Novosphingobium pentaromativorans US6-1]
VPRFHLTRAAADDLTAIFLEGIEQFGLPQADAYHEGLSAIFAFLADYPHAARLREEISPPVRVHPYKAHLVIYDVGNEGE
VIILRVRHGREDWTSSNYDG

Nucleotide

• Length: 303 bp

>T48749 NZ_CP009292:389665-389967 [Novosphingobium pentaromativorans US6-1]
GTGCCACGTTTTCACTTAACACGCGCCGCAGCCGACGATCTGACAGCCATTTTTCTGGAAGGCATCGAGCAGTTTGGCTT
GCCGCAGGCTGACGCTTATCACGAAGGGCTGAGTGCGATTTTCGCGTTTCTCGCAGACTATCCCCATGCCGCGCGCTTGC
GAGAGGAAATTTCGCCGCCAGTTCGCGTGCATCCCTACAAGGCACACCTGGTGATTTATGATGTAGGAAATGAGGGCGAG
GTCATCATTCTACGCGTCCGGCATGGTCGGGAAGATTGGACATCATCGAATTACGACGGTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8996.07 Da
• Isoelectric Point: 4.7107

>AT48749 WP_039858067.1 NZ_CP009292:389417-389662 [Novosphingobium pentaromativorans US6-1]
MATMNISLPDPMKQWVEAQADTGRYSNASDYVRDLIRRDQERADKIAAMQRLVDDARAGGLSDETMADIRARAINQAGLQ
A

Nucleotide

• Length: 246 bp

>AT48749 NZ_CP009292:389417-389662 [Novosphingobium pentaromativorans US6-1]
ATGGCTACGATGAACATCTCACTGCCCGATCCGATGAAGCAATGGGTGGAAGCGCAAGCTGATACTGGTCGGTATAGCAA
TGCCAGCGACTATGTGCGCGATCTTATCCGCCGTGACCAGGAACGCGCCGACAAGATTGCCGCCATGCAGCGCCTTGTCG
ACGACGCCCGAGCTGGCGGTCTGAGCGATGAGACGATGGCGGATATCCGGGCGCGAGCGATCAACCAGGCAGGCCTGCAA
GCCTGA

Structures

Toxin

• AlphaFold DB: G6EGT7