Detailed information of TA system TA048744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 6296468..6297148
• Replicon: chromosome
• Accession: NZ_CP009290
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain JD37

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A285GR45
• Locus tag: JM49_RS28235
• Protein ID: WP_028681531.1
• Coordinates: 6296468..6296650 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JM49_RS28240
• Protein ID: WP_038582934.1
• Coordinates: 6296723..6297148 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JM49_RS28205	6291790..6292500	+	711	WP_038582925.1	hypothetical protein	-
JM49_RS28210	6292497..6293420	+	924	WP_038584004.1	hypothetical protein	-
JM49_RS28215	6293417..6293959	+	543	WP_038582927.1	hypothetical protein	-
JM49_RS28220	6293956..6294660	+	705	WP_038582929.1	hypothetical protein	-
JM49_RS28225	6294633..6295604	+	972	WP_038582931.1	hypothetical protein	-
JM49_RS28230	6295601..6296242	-	642	WP_052107453.1	hypothetical protein	-
JM49_RS28235	6296468..6296650	+	183	WP_028681531.1	type II toxin-antitoxin system HicA family toxin	Toxin
JM49_RS28240	6296723..6297148	+	426	WP_038582934.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JM49_RS31255	6298115..6299191	+	1077	WP_155290757.1	hypothetical protein	-
JM49_RS31260	6300019..6300348	-	330	WP_155290758.1	hypothetical protein	-
JM49_RS31265	6300348..6301175	-	828	WP_155290759.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	6261988..6297148	35160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6764.83 Da
• Isoelectric Point: 10.7304

>T48744 WP_028681531.1 NZ_CP009290:6296468-6296650 [Pseudomonas chlororaphis subsp. aurantiaca]
MKYSEFRRWLKAQGAEFQPGKGSHFKVTLNGKSTVFPDHGAKEMGEGLRKSIIKQLGLKD

Nucleotide

• Length: 183 bp

>T48744 NZ_CP009290:6296468-6296650 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGAAGTACAGCGAGTTTCGGCGATGGTTGAAAGCCCAAGGCGCGGAGTTCCAACCCGGCAAGGGCAGTCACTTCAAGGT
CACCTTGAATGGCAAGTCAACCGTGTTTCCAGACCACGGAGCCAAGGAAATGGGCGAAGGGTTAAGGAAGTCGATAATCA
AACAGCTGGGCCTCAAGGATTGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15405.57 Da
• Isoelectric Point: 4.7124

>AT48744 WP_038582934.1 NZ_CP009290:6296723-6297148 [Pseudomonas chlororaphis subsp. aurantiaca]
MFEYALEVHDEPGSVWLSCAEIPEMHAAGDTLGEALDGALDSIETALSIYVDERRAIPAGEPGAKAGHIVLRLPALTAAK
IALWNTLLESGMSKAELARRLDVQRPQVDRLVDFLHHSKIENVERALQELGRRISITVEAA

Nucleotide

• Length: 426 bp

>AT48744 NZ_CP009290:6296723-6297148 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGTTTGAGTATGCGTTAGAGGTTCACGATGAACCGGGCAGCGTTTGGTTGTCATGTGCAGAAATCCCAGAGATGCACGC
CGCCGGCGACACTCTCGGGGAAGCCCTGGACGGTGCCCTTGATTCGATTGAGACAGCATTGTCCATCTACGTCGATGAGC
GCCGGGCCATTCCCGCCGGTGAACCAGGTGCGAAAGCGGGCCATATCGTTCTCCGTCTGCCTGCACTGACTGCGGCGAAG
ATTGCGCTGTGGAATACGCTCCTGGAGTCTGGAATGAGCAAAGCCGAGTTGGCACGCCGCCTGGACGTACAGCGGCCCCA
AGTGGATCGCTTGGTCGATTTTCTACACCACTCCAAGATTGAGAACGTCGAGCGCGCCCTGCAGGAACTCGGTCGGCGGA
TCTCTATCACCGTCGAAGCTGCATAA

Structures

Toxin

• AlphaFold DB: A0A285GR45