Detailed information of TA system TA048740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1356599..1357227
• Replicon: chromosome
• Accession: NZ_CP009290
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain JD37

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JM49_RS06255
• Protein ID: WP_038576104.1
• Coordinates: 1356829..1357227 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JM49_RS06250
• Protein ID: WP_038576101.1
• Coordinates: 1356599..1356829 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JM49_RS06240	1351934..1355347	-	3414	WP_038576096.1	type I-F CRISPR-associated helicase Cas3	-
JM49_RS06245	1355344..1356321	-	978	WP_038576099.1	type I-F CRISPR-associated endonuclease Cas1	-
JM49_RS06250	1356599..1356829	+	231	WP_038576101.1	antitoxin	Antitoxin
JM49_RS06255	1356829..1357227	+	399	WP_038576104.1	tRNA(fMet)-specific endonuclease VapC	Toxin
JM49_RS06260	1357285..1359234	-	1950	WP_038576106.1	alkaline phosphatase family protein	-
JM49_RS06265	1359373..1359729	-	357	WP_080748359.1	hypothetical protein	-
JM49_RS06270	1359900..1360634	-	735	WP_038576111.1	alpha/beta hydrolase	-
JM49_RS06275	1360638..1361135	-	498	WP_038583466.1	PAAR domain-containing protein	-
JM49_RS06280	1361723..1361998	-	276	WP_038576112.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14886.17 Da
• Isoelectric Point: 7.2433

>T48740 WP_038576104.1 NZ_CP009290:1356829-1357227 [Pseudomonas chlororaphis subsp. aurantiaca]
MLKYMLDTNICIFTIKNKPERVREAFNRQHGRLCISSVTLMELIYGAEKSAAPERNLSVIEGFVARLEVLAYDYEAAIHS
GQLRAELARAGTPIGPYDQLIAGHARSQGLVLVTNNVREFERVPGLRIEDWL

Nucleotide

• Length: 399 bp

>T48740 NZ_CP009290:1356829-1357227 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCTCAAGTACATGTTGGATACGAACATCTGTATTTTCACCATCAAGAACAAACCGGAGCGGGTTCGGGAGGCGTTCAA
CCGCCAGCACGGCCGGTTGTGCATCAGTTCGGTGACCTTGATGGAACTAATTTATGGAGCAGAAAAATCAGCGGCTCCTG
AGAGAAACCTGTCTGTCATAGAGGGCTTCGTGGCACGTCTGGAAGTGCTCGCGTATGACTACGAAGCTGCTATTCACAGC
GGTCAGTTGCGTGCCGAGTTGGCCAGGGCTGGTACGCCAATAGGTCCTTACGATCAGCTGATTGCCGGCCATGCCAGATC
CCAGGGATTGGTATTGGTGACCAACAATGTGCGCGAGTTTGAACGGGTGCCAGGGCTGCGTATTGAGGATTGGCTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8487.41 Da
• Isoelectric Point: 4.2821

>AT48740 WP_038576101.1 NZ_CP009290:1356599-1356829 [Pseudomonas chlororaphis subsp. aurantiaca]
MEQGSVFKSNRSQAIRMPKAVALPDDVTRVDIVAVGRTRIITPAGEAWDSWFDGESVSADFMSERDQPAEQEREAF

Nucleotide

• Length: 231 bp

>AT48740 NZ_CP009290:1356599-1356829 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGGAACAAGGGTCGGTATTCAAAAGTAACCGCAGCCAGGCCATTCGAATGCCCAAGGCAGTGGCTCTCCCTGACGATGT
AACGCGGGTGGATATCGTTGCGGTAGGGCGTACCCGGATCATTACCCCGGCGGGGGAAGCCTGGGACAGCTGGTTCGATG
GCGAGTCGGTGAGCGCGGACTTCATGAGTGAGCGGGATCAGCCTGCGGAACAAGAGCGTGAGGCGTTCTGA