Detailed information of TA system TA048737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1463468..1464073
• Replicon: chromosome
• Accession: NZ_CP009287
• Organism: Paenibacillus graminis strain DSM 15220

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0E4H7G2
• Locus tag: PGRAT_RS06250
• Protein ID: WP_019913013.1
• Coordinates: 1463723..1464073 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PGRAT_RS06245
• Protein ID: WP_174469047.1
• Coordinates: 1463468..1463719 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PGRAT_RS06225	1459236..1460072	+	837	WP_025708960.1	hypothetical protein	-
PGRAT_RS06230	1460136..1460555	+	420	WP_155990556.1	hypothetical protein	-
PGRAT_RS06235	1460737..1461822	+	1086	WP_025708962.1	outer membrane lipoprotein carrier protein LolA	-
PGRAT_RS06240	1462045..1463226	+	1182	WP_025708963.1	alanine racemase	-
PGRAT_RS06245	1463468..1463719	+	252	WP_174469047.1	ribbon-helix-helix protein, CopG family	Antitoxin
PGRAT_RS06250	1463723..1464073	+	351	WP_019913013.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PGRAT_RS06255	1464486..1465790	+	1305	WP_025708965.1	extracellular solute-binding protein	-
PGRAT_RS06260	1465897..1466781	+	885	WP_025708966.1	sugar ABC transporter permease	-
PGRAT_RS06265	1466784..1467611	+	828	WP_025708967.1	carbohydrate ABC transporter permease	-
PGRAT_RS06270	1467637..1468455	+	819	WP_025708968.1	carbohydrate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-35)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12749.67 Da
• Isoelectric Point: 4.8265

>T48737 WP_019913013.1 NZ_CP009287:1463723-1464073 [Paenibacillus graminis]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDETMKLVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T48737 NZ_CP009287:1463723-1464073 [Paenibacillus graminis]
TTGATCGTTAAACGCGGCGACGTTTTTTTTGCCGACCTTTCGCCGGTGGTGGGTTCAGAACAAGGCGGAGTACGGCCGGT
ACTGGTCATTCAGAACGACATTGGCAATCGCTTCAGCCCGACAGTCATTGTGGCAGCCATCACTGCCCAGATTCAGAAGG
CTAAACTGCCGACGCATGTAGAAATTGATGCGGCTGCTCACGGCTTTGACAGGGACTCCGTCATATTGCTGGAGCAGGTT
CGGACAATTGACAAACAACGCTTAACCGATAAGATCACCCATCTGGACGATGAAACAATGAAGCTGGTGGATGATTCGCT
GCAAATCAGCCTGGGACTGATTGATTTTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9487.94 Da
• Isoelectric Point: 5.1051

>AT48737 WP_174469047.1 NZ_CP009287:1463468-1463719 [Paenibacillus graminis]
MISLPDHLLQEVDGIVQLENSNRSELIRQAMKLYLSERRKRTIRDSMQRGYMEMAKINLTMACEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT48737 NZ_CP009287:1463468-1463719 [Paenibacillus graminis]
ATGATCAGCTTGCCCGATCATCTCTTGCAGGAAGTGGATGGGATCGTCCAATTGGAGAACTCCAACCGGAGTGAATTGAT
CAGGCAGGCCATGAAGCTGTATCTAAGCGAACGGAGGAAACGGACCATCCGCGATTCGATGCAGCGTGGCTATATGGAAA
TGGCTAAAATCAATTTGACCATGGCATGCGAGGCGTTTCTCGCGGAGGAAGATGCAGACAGTACTCTTGGCCGCTTAGTA
AGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A0E4H7G2