Detailed information of TA system TA048736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1362710..1363315
• Replicon: chromosome
• Accession: NZ_CP009286
• Organism: Paenibacillus stellifer strain DSM 14472

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: X4ZF21
• Locus tag: PSTEL_RS06195
• Protein ID: WP_025333625.1
• Coordinates: 1362965..1363315 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PSTEL_RS06190
• Protein ID: WP_179944928.1
• Coordinates: 1362710..1362961 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSTEL_RS06165	1357740..1358324	+	585	WP_038694218.1	sigma-70 family RNA polymerase sigma factor	-
PSTEL_RS06170	1358314..1359045	+	732	WP_052098227.1	DUF3600 domain-containing protein	-
PSTEL_RS06175	1359142..1359705	-	564	WP_038694221.1	CueP family metal-binding protein	-
PSTEL_RS06180	1359961..1361046	+	1086	WP_038694223.1	outer membrane lipoprotein carrier protein LolA	-
PSTEL_RS06185	1361284..1362486	+	1203	WP_038694224.1	alanine racemase	-
PSTEL_RS06190	1362710..1362961	+	252	WP_179944928.1	ribbon-helix-helix protein, CopG family	Antitoxin
PSTEL_RS06195	1362965..1363315	+	351	WP_025333625.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PSTEL_RS06205	1363804..1366020	+	2217	WP_052098228.1	RNA-binding transcriptional accessory protein	-
PSTEL_RS06210	1366147..1367118	-	972	WP_038694231.1	S-layer homology domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-35)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12794.71 Da
• Isoelectric Point: 5.1284

>T48736 WP_025333625.1 NZ_CP009286:1362965-1363315 [Paenibacillus stellifer]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIEAASHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDETMKKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T48736 NZ_CP009286:1362965-1363315 [Paenibacillus stellifer]
TTGATCGTAAAACGCGGCGACGTTTTTTTTGCCGATCTTTCGCCGGTGGTGGGTTCCGAGCAGGGCGGTGTCCGACCCGT
CCTCGTCATTCAGAACGATATTGGCAATCGCTTTAGTCCGACGGTAATCGTTGCGGCGATTACCGCACAGATCCAGAAGG
CCAAGCTACCTACTCATGTGGAGATTGAGGCAGCCTCCCACGGATTCGACAGGGATTCCGTGATTCTCCTGGAACAAGTC
CGGACCATCGACAAGCAGCGCCTAACGGACAAGATAACCCACCTGGACGACGAAACGATGAAAAAGGTGGATGATTCGCT
GCAAATCAGCCTGGGTTTGATTGATTTTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9488.93 Da
• Isoelectric Point: 4.8355

>AT48736 WP_179944928.1 NZ_CP009286:1362710-1362961 [Paenibacillus stellifer]
MISLPDHLLQEVDGIVELENSNRSELIRQAMKLYLSERRKRSIRESMQRGYMEMAKINLTMACEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT48736 NZ_CP009286:1362710-1362961 [Paenibacillus stellifer]
ATGATTAGCTTGCCCGATCATCTCTTGCAAGAGGTGGATGGAATTGTCGAACTGGAGAATTCCAACCGCAGCGAATTGAT
TCGGCAGGCCATGAAGCTGTATCTTAGCGAACGGAGGAAGCGTTCCATAAGGGAGTCCATGCAGCGCGGATACATGGAGA
TGGCCAAGATCAACTTGACCATGGCATGTGAAGCGTTCCTAGCCGAGGAAGATGCAGACAGTACTCTTGGCCGCTTAGTA
AGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: X4ZF21