Detailed information of TA system TA048735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1469405..1470010
• Replicon: chromosome
• Accession: NZ_CP009285
• Organism: Paenibacillus borealis strain DSM 13188

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0E4H7G2
• Locus tag: PBOR_RS06245
• Protein ID: WP_019913013.1
• Coordinates: 1469660..1470010 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PBOR_RS06240
• Protein ID: WP_171717509.1
• Coordinates: 1469405..1469656 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PBOR_RS06220	1464681..1465244	+	564	WP_042210937.1	sigma-70 family RNA polymerase sigma factor	-
PBOR_RS06225	1465241..1466140	+	900	WP_042210938.1	DUF4367 domain-containing protein	-
PBOR_RS06230	1466718..1467800	+	1083	WP_042210939.1	outer membrane lipoprotein carrier protein LolA	-
PBOR_RS06235	1467983..1469164	+	1182	WP_042210940.1	alanine racemase	-
PBOR_RS06240	1469405..1469656	+	252	WP_171717509.1	ribbon-helix-helix protein, CopG family	Antitoxin
PBOR_RS06245	1469660..1470010	+	351	WP_019913013.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PBOR_RS37550	1470029..1470352	+	324	Protein_1199	helix-turn-helix transcriptional regulator	-
PBOR_RS06255	1471130..1472338	+	1209	WP_042210942.1	tetracycline resistance MFS efflux pump	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12749.67 Da
• Isoelectric Point: 4.8265

>T48735 WP_019913013.1 NZ_CP009285:1469660-1470010 [Paenibacillus borealis]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDETMKLVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T48735 NZ_CP009285:1469660-1470010 [Paenibacillus borealis]
TTGATTGTTAAACGCGGCGACGTTTTTTTTGCCGACCTTTCACCGGTCGTGGGTTCAGAGCAAGGCGGAGTTAGGCCGGT
ACTGGTCATTCAGAACGACATTGGCAATCGCTTCAGCCCGACTGTAATTGTGGCAGCTATCACTGCCCAGATCCAGAAGG
CCAAACTGCCGACGCATGTGGAAATTGATGCGGCTGCCCATGGCTTTGACCGGGACTCCGTTATATTGCTGGAGCAGGTT
CGGACCATTGACAAACAACGCTTAACCGATAAGATCACCCACCTGGATGACGAAACCATGAAACTGGTGGATGATTCGCT
GCAAATCAGCCTGGGTTTGATTGATTTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9499.94 Da
• Isoelectric Point: 5.1186

>AT48735 WP_171717509.1 NZ_CP009285:1469405-1469656 [Paenibacillus borealis]
MISLPDHLLQEVDGIVQLENSNRSELIRQAMKLYLTERRKRTIRESMQRGYMEMAKINLTMASEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT48735 NZ_CP009285:1469405-1469656 [Paenibacillus borealis]
ATGATCAGCTTGCCCGATCATCTCTTACAGGAAGTGGATGGGATCGTTCAATTGGAGAACTCTAACCGTAGTGAATTGAT
CAGGCAGGCCATGAAGCTGTACTTGACTGAACGGAGGAAACGAACCATCCGGGAGTCGATGCAGCGGGGATACATGGAAA
TGGCTAAGATTAATTTGACCATGGCAAGCGAGGCCTTTCTCGCAGAGGAAGATGCAGACAGTACTCTTGGCCGCTTAGTA
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0E4H7G2