Detailed information of TA system TA048733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1256400..1257005
• Replicon: chromosome
• Accession: NZ_CP009284
• Organism: Paenibacillus sp. FSL R7-0331

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1G8TMW4
• Locus tag: R70331_RS05340
• Protein ID: WP_039870351.1
• Coordinates: 1256655..1257005 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: R70331_RS05335
• Protein ID: WP_179088072.1
• Coordinates: 1256400..1256651 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R70331_RS05315	1251430..1252272	+	843	WP_042173495.1	hypothetical protein	-
R70331_RS05320	1252282..1253499	+	1218	WP_042173497.1	hypothetical protein	-
R70331_RS05325	1253664..1254746	+	1083	WP_042173499.1	outer membrane lipoprotein carrier protein LolA	-
R70331_RS05330	1254977..1256158	+	1182	WP_042173501.1	alanine racemase	-
R70331_RS05335	1256400..1256651	+	252	WP_179088072.1	ribbon-helix-helix protein, CopG family	Antitoxin
R70331_RS05340	1256655..1257005	+	351	WP_039870351.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
R70331_RS30340	1257212..1257766	+	555	WP_052418931.1	TetR/AcrR family transcriptional regulator	-
R70331_RS05350	1258009..1259217	+	1209	WP_042173503.1	tetracycline resistance MFS efflux pump	-
R70331_RS05355	1259368..1261566	+	2199	WP_197071445.1	RNA-binding transcriptional accessory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12750.66 Da
• Isoelectric Point: 5.1057

>T48733 WP_039870351.1 NZ_CP009284:1256655-1257005 [Paenibacillus sp. FSL R7-0331]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDDTMKKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T48733 NZ_CP009284:1256655-1257005 [Paenibacillus sp. FSL R7-0331]
TTGATCGTTAAACGCGGCGACGTTTTTTTTGCCGACCTTTCGCCGGTTGTAGGCTCTGAACAAGGCGGAGTAAGGCCGGT
ACTGGTCATACAGAACGATATTGGCAACCGGTTCAGCCCGACGGTTATTGTAGCAGCTATTACTGCCCAGATCCAGAAGG
CCAAACTGCCGACGCATGTGGAGATTGATGCGGCTGCCCACGGCTTTGACCGGGATTCCGTCATTTTGCTGGAGCAGGTT
CGGACGATTGACAAACAACGCTTAACTGATAAGATCACCCATCTGGACGATGATACAATGAAAAAGGTGGATGATTCGCT
GCAAATCAGTCTGGGCCTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9471.88 Da
• Isoelectric Point: 5.1186

>AT48733 WP_179088072.1 NZ_CP009284:1256400-1256651 [Paenibacillus sp. FSL R7-0331]
MISLPDHLLQEVDGIAQLENSNRSELIRQAMKLYLTERRKRTIRESMQRGYMEMAKINLTMASEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT48733 NZ_CP009284:1256400-1256651 [Paenibacillus sp. FSL R7-0331]
ATGATCAGTTTGCCCGATCATCTTTTGCAGGAAGTGGATGGGATCGCCCAATTGGAGAACTCTAACCGGAGTGAATTGAT
CAGGCAGGCCATGAAGCTGTATCTGACGGAACGCAGAAAGCGTACAATCCGGGAATCGATGCAGCGGGGATACATGGAGA
TGGCAAAGATCAATTTGACAATGGCAAGCGAGGCATTTCTCGCGGAGGAAGATGCAGACAGTACTCTTGGCCGCTTAGTA
AGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A1G8TMW4