Detailed information of TA system TA048731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1234460..1235065
• Replicon: chromosome
• Accession: NZ_CP009283
• Organism: Paenibacillus sp. FSL R7-0273

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1G8TMW4
• Locus tag: R70723_RS05315
• Protein ID: WP_039870351.1
• Coordinates: 1234715..1235065 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: R70723_RS05310
• Protein ID: WP_179088072.1
• Coordinates: 1234460..1234711 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R70723_RS32760	1229850..1230032	-	183	WP_076418452.1	hypothetical protein	-
R70723_RS05295	1230289..1231500	+	1212	WP_039870347.1	hypothetical protein	-
R70723_RS05300	1231727..1232809	+	1083	WP_039870348.1	outer membrane lipoprotein-sorting protein	-
R70723_RS05305	1233037..1234218	+	1182	WP_039870350.1	alanine racemase	-
R70723_RS05310	1234460..1234711	+	252	WP_179088072.1	ribbon-helix-helix protein, CopG family	Antitoxin
R70723_RS05315	1234715..1235065	+	351	WP_039870351.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
R70723_RS31890	1235271..1235825	+	555	WP_052421188.1	TetR/AcrR family transcriptional regulator	-
R70723_RS05325	1236045..1237253	+	1209	WP_039870353.1	tetracycline resistance MFS efflux pump	-
R70723_RS05330	1237403..1239601	+	2199	WP_179088071.1	RNA-binding transcriptional accessory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12750.66 Da
• Isoelectric Point: 5.1057

>T48731 WP_039870351.1 NZ_CP009283:1234715-1235065 [Paenibacillus sp. FSL R7-0273]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDDTMKKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T48731 NZ_CP009283:1234715-1235065 [Paenibacillus sp. FSL R7-0273]
TTGATCGTTAAACGCGGCGACGTTTTTTTTGCCGACCTTTCGCCGGTTGTAGGCTCTGAACAAGGCGGAGTAAGGCCGGT
ACTGGTCATACAGAACGATATTGGCAACCGGTTCAGCCCGACGGTTATTGTGGCAGCTATTACTGCCCAGATCCAGAAGG
CCAAGCTGCCGACGCATGTAGAGATTGATGCGGCTGCTCACGGCTTTGACCGGGATTCCGTCATTTTGCTGGAGCAGGTT
CGGACGATTGACAAACAACGCTTAACCGATAAGATCACCCATCTGGACGATGATACAATGAAAAAGGTGGATGATTCGCT
GCAAATCAGTCTGGGCCTGATCGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9471.88 Da
• Isoelectric Point: 5.1186

>AT48731 WP_179088072.1 NZ_CP009283:1234460-1234711 [Paenibacillus sp. FSL R7-0273]
MISLPDHLLQEVDGIAQLENSNRSELIRQAMKLYLTERRKRTIRESMQRGYMEMAKINLTMASEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT48731 NZ_CP009283:1234460-1234711 [Paenibacillus sp. FSL R7-0273]
ATGATCAGTTTGCCCGATCATCTTTTGCAGGAAGTGGATGGGATCGCCCAATTGGAGAACTCTAACCGGAGTGAATTGAT
CAGGCAGGCCATGAAGCTGTATCTGACGGAACGCAGAAAGCGTACAATCCGGGAATCGATGCAGCGGGGATACATGGAGA
TGGCCAAGATCAATTTGACGATGGCAAGCGAGGCATTTCTCGCGGAGGAAGATGCAGACAGTACTCTTGGCCGCTTAGTA
AGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A1G8TMW4