Detailed information of TA system TA048730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1336960..1337565
• Replicon: chromosome
• Accession: NZ_CP009282
• Organism: Paenibacillus sp. FSL R5-0912

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0E4H7G2
• Locus tag: R50912_RS05785
• Protein ID: WP_019913013.1
• Coordinates: 1337215..1337565 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: R50912_RS05780
• Protein ID: WP_171717509.1
• Coordinates: 1336960..1337211 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R50912_RS05770	1334275..1335357	+	1083	WP_042233121.1	outer membrane lipoprotein carrier protein LolA	-
R50912_RS05775	1335538..1336719	+	1182	WP_042233123.1	alanine racemase	-
R50912_RS05780	1336960..1337211	+	252	WP_171717509.1	ribbon-helix-helix protein, CopG family	Antitoxin
R50912_RS05785	1337215..1337565	+	351	WP_019913013.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
R50912_RS05790	1337857..1338402	+	546	WP_081956390.1	TetR/AcrR family transcriptional regulator	-
R50912_RS05795	1338723..1339934	+	1212	WP_042233126.1	tetracycline resistance MFS efflux pump	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12749.67 Da
• Isoelectric Point: 4.8265

>T48730 WP_019913013.1 NZ_CP009282:1337215-1337565 [Paenibacillus sp. FSL R5-0912]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDETMKLVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T48730 NZ_CP009282:1337215-1337565 [Paenibacillus sp. FSL R5-0912]
TTGATTGTTAAACGCGGCGACGTTTTTTTTGCCGACCTTTCACCGGTCGTGGGTTCAGAGCAAGGCGGAGTTAGGCCGGT
ACTGGTCATTCAGAACGACATTGGCAATCGCTTCAGCCCGACTGTAATTGTGGCAGCTATCACTGCCCAGATCCAGAAGG
CCAAACTGCCGACGCATGTGGAGATTGATGCGGCTGCCCATGGCTTTGACCGGGATTCCGTTATATTGCTGGAGCAGGTT
CGGACCATTGACAAACAACGCTTAACCGATAAGATCACCCACTTGGACGATGAGACCATGAAGCTGGTGGATGATTCGCT
GCAAATCAGCCTGGGTCTGATTGACTTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9499.94 Da
• Isoelectric Point: 5.1186

>AT48730 WP_171717509.1 NZ_CP009282:1336960-1337211 [Paenibacillus sp. FSL R5-0912]
MISLPDHLLQEVDGIVQLENSNRSELIRQAMKLYLTERRKRTIRESMQRGYMEMAKINLTMASEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT48730 NZ_CP009282:1336960-1337211 [Paenibacillus sp. FSL R5-0912]
ATGATCAGCTTGCCCGATCATCTCTTGCAGGAAGTGGATGGGATCGTTCAATTGGAGAACTCTAACCGTAGTGAATTGAT
CAGGCAGGCCATGAAGCTGTACTTGACTGAACGGAGGAAACGTACCATCCGGGAGTCGATGCAGCGGGGATACATGGAAA
TGGCTAAGATTAATTTGACCATGGCAAGCGAGGCCTTTCTCGCAGAGGAAGATGCAGACAGTACTCTTGGCCGCTTAGTA
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0E4H7G2