Detailed information of TA system TA048729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1233135..1233740
• Replicon: chromosome
• Accession: NZ_CP009281
• Organism: Paenibacillus sp. FSL R5-0345

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0W1B4L6
• Locus tag: R50345_RS05410
• Protein ID: WP_042124727.1
• Coordinates: 1233390..1233740 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: R50345_RS05405
• Protein ID: WP_154117276.1
• Coordinates: 1233135..1233386 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R50345_RS05385	1228506..1229906	+	1401	WP_042131890.1	helix-turn-helix transcriptional regulator	-
R50345_RS05390	1229994..1230167	+	174	WP_197069747.1	aspartyl-phosphate phosphatase Spo0E family protein	-
R50345_RS05395	1230402..1231487	+	1086	WP_042124723.1	outer membrane lipoprotein carrier protein LolA	-
R50345_RS05400	1231709..1232890	+	1182	WP_042124725.1	alanine racemase	-
R50345_RS05405	1233135..1233386	+	252	WP_154117276.1	ribbon-helix-helix protein, CopG family	Antitoxin
R50345_RS05410	1233390..1233740	+	351	WP_042124727.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
R50345_RS05415	1233897..1234505	+	609	WP_052414476.1	TetR/AcrR family transcriptional regulator	-
R50345_RS05420	1234682..1235881	+	1200	WP_042124729.1	MFS transporter	-
R50345_RS05425	1236066..1238228	+	2163	WP_197069795.1	RNA-binding transcriptional accessory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-35)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12764.68 Da
• Isoelectric Point: 5.1171

>T48729 WP_042124727.1 NZ_CP009281:1233390-1233740 [Paenibacillus sp. FSL R5-0345]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDETMKKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T48729 NZ_CP009281:1233390-1233740 [Paenibacillus sp. FSL R5-0345]
TTGATCGTTAAACGTGGCGATGTTTTTTTTGCTGATCTTTCGCCGGTTGTAGGTTCTGAGCAAGGCGGAGTAAGGCCAGT
ACTGGTTATCCAGAATGATATTGGCAATCGCTTTAGCCCGACGGTAATTGTGGCTGCAATCACAGCCCAAATCCAGAAGG
CTAAATTACCTACGCATGTGGAAATTGACGCAGCGGCTCATGGCTTTGACAGGGATTCTGTTATACTGCTGGAGCAGGTT
AGGACGATTGACAAACAACGCTTAACTGACAAGATCACCCACCTTGATGATGAAACGATGAAGAAGGTGGATGATTCGCT
GCAAATCAGCCTTGGACTGATTGATTTTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9487.94 Da
• Isoelectric Point: 5.1184

>AT48729 WP_154117276.1 NZ_CP009281:1233135-1233386 [Paenibacillus sp. FSL R5-0345]
MISLPDHLLQEVDGIVQLENSNRSELIRQAMKLYLSERRKRSIRESMQRGYMEMAKINLTMACEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT48729 NZ_CP009281:1233135-1233386 [Paenibacillus sp. FSL R5-0345]
ATGATCAGCTTGCCCGATCATCTCTTACAGGAAGTGGACGGAATTGTTCAATTGGAGAATTCCAACCGGAGTGAATTGAT
TAGGCAGGCCATGAAGCTGTATTTGAGCGAACGGAGGAAACGTTCTATCCGGGAGTCCATGCAGCGCGGCTACATGGAAA
TGGCTAAGATCAACTTGACCATGGCATGCGAGGCGTTCCTCGCGGAGGAAGATGCAGACAGCACTCTTGGCCGCTTAGTA
AGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A0W1B4L6