Detailed information of TA system TA048725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 79011..79750
• Replicon: plasmid pKV1
• Accession: NZ_CP009275
• Organism: Klebsiella variicola strain DX120E

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A5E1AVR5
• Locus tag: KR75_RS26220
• Protein ID: WP_013087172.1
• Coordinates: 79265..79750 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A5E1AWX8
• Locus tag: KR75_RS26215
• Protein ID: WP_012540086.1
• Coordinates: 79011..79277 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KR75_RS26190	74242..74778	-	537	WP_013087166.1	GNAT family N-acetyltransferase	-
KR75_RS26195	74903..76654	-	1752	WP_203227027.1	arsenical pump-driving ATPase	-
KR75_RS26200	76681..77043	-	363	WP_013087168.1	arsenic metallochaperone ArsD family protein	-
KR75_RS26205	77119..77664	-	546	WP_040107919.1	sigma-70 family RNA polymerase sigma factor	-
KR75_RS26210	77673..78386	-	714	WP_013087170.1	arsenical resistance protein ArsH	-
KR75_RS28205	78388..78711	-	324	WP_065907728.1	metalloregulator ArsR/SmtB family transcription factor	-
KR75_RS26215	79011..79277	+	267	WP_012540086.1	DUF1778 domain-containing protein	Antitoxin
KR75_RS26220	79265..79750	+	486	WP_013087172.1	GNAT family N-acetyltransferase	Toxin
KR75_RS26225	80092..80250	+	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
KR75_RS26230	81154..81534	+	381	WP_038990973.1	hypothetical protein	-
KR75_RS26235	81601..81948	+	348	WP_038990975.1	hypothetical protein	-
KR75_RS28210	82036..82266	+	231	WP_038990977.1	hypothetical protein	-
KR75_RS26245	82313..83146	+	834	WP_040107920.1	type I restriction-modification system subunit M	-
KR75_RS26250	83771..84301	+	531	WP_040107921.1	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..162706	162706

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17810.62 Da
• Isoelectric Point: 9.8560

>T48725 WP_013087172.1 NZ_CP009275:79265-79750 [Klebsiella variicola]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T48725 NZ_CP009275:79265-79750 [Klebsiella variicola]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10076.47 Da
• Isoelectric Point: 4.3721

>AT48725 WP_012540086.1 NZ_CP009275:79011-79277 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT48725 NZ_CP009275:79011-79277 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A5E1AVR5

Antitoxin

• AlphaFold DB: A0A5E1AWX8