Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4235785..4236404 Replicon chromosome
Accession NZ_CP009274
Organism Klebsiella variicola strain DX120E

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KR75_RS19960 Protein ID WP_002892050.1
Coordinates 4236186..4236404 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag KR75_RS19955 Protein ID WP_008805436.1
Coordinates 4235785..4236159 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KR75_RS19945 4230940..4232133 + 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KR75_RS19950 4232156..4235302 + 3147 WP_012968813.1 multidrug efflux RND transporter permease subunit -
KR75_RS19955 4235785..4236159 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
KR75_RS19960 4236186..4236404 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
KR75_RS19965 4236563..4237129 + 567 WP_039102911.1 maltose O-acetyltransferase -
KR75_RS29785 4237101..4237229 - 129 Protein_4047 hypothetical protein -
KR75_RS19970 4237266..4237736 + 471 WP_008805434.1 YlaC family protein -
KR75_RS19975 4237705..4239162 - 1458 WP_039102910.1 PLP-dependent aminotransferase family protein -
KR75_RS19980 4239263..4239961 + 699 WP_016160391.1 GNAT family N-acetyltransferase -
KR75_RS19985 4239958..4240098 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KR75_RS19990 4240098..4240361 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T48722 WP_002892050.1 NZ_CP009274:4236186-4236404 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T48722 NZ_CP009274:4236186-4236404 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT48722 WP_008805436.1 NZ_CP009274:4235785-4236159 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT48722 NZ_CP009274:4235785-4236159 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References