48670

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 475546..476164 Replicon chromosome
Accession NZ_CP009273
Organism Escherichia coli BW25113

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BW25113_RS02375 Protein ID WP_001291435.1
Coordinates 475546..475764 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BW25113_RS02380 Protein ID WP_000344800.1
Coordinates 475790..476164 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BW25113_RS02345 (BW25113_0453) 470835..471407 + 573 WP_000779842.1 YbaY family lipoprotein -
BW25113_RS02350 (BW25113_0454) 471438..471749 - 312 WP_000409911.1 MGMT family protein -
BW25113_RS02355 (BW25113_0456) 472128..472481 + 354 WP_000878140.1 DUF1428 domain-containing protein -
BW25113_RS02360 (BW25113_0457) 472523..474073 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BW25113_RS02365 (BW25113_0458) 474237..474707 - 471 WP_000136192.1 YlaC family protein -
BW25113_RS02370 (BW25113_0459) 474823..475374 - 552 WP_000102564.1 maltose O-acetyltransferase -
BW25113_RS02375 (BW25113_0460) 475546..475764 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BW25113_RS02380 (BW25113_0461) 475790..476164 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BW25113_RS02385 (BW25113_0462) 476710..479859 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
BW25113_RS02390 (BW25113_0463) 479882..481075 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T48670 WP_001291435.1 NZ_CP009273:c475764-475546 [Escherichia coli BW25113]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T48670 NZ_CP009273:c475764-475546 [Escherichia coli BW25113]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT48670 WP_000344800.1 NZ_CP009273:c476164-475790 [Escherichia coli BW25113]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT48670 NZ_CP009273:c476164-475790 [Escherichia coli BW25113]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References