Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 259039..259718 | Replicon | chromosome |
| Accession | NZ_CP009273 | ||
| Organism | Escherichia coli BW25113 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | BW25113_RS01240 | Protein ID | WP_000854672.1 |
| Coordinates | 259039..259380 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | BW25113_RS01245 | Protein ID | WP_000070395.1 |
| Coordinates | 259401..259718 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BW25113_RS01215 (BW25113_0240) | 254316..254717 | + | 402 | WP_000174689.1 | sigma factor-binding protein Crl | - |
| BW25113_RS01220 (BW25113_0241) | 254756..255811 | - | 1056 | WP_000749863.1 | phosphoporin PhoE | - |
| BW25113_RS01225 (BW25113_0242) | 256099..257202 | + | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| BW25113_RS01230 (BW25113_0243) | 257214..258467 | + | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| BW25113_RS01240 (BW25113_0245) | 259039..259380 | - | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| BW25113_RS01245 (BW25113_0246) | 259401..259718 | - | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| BW25113_RS23240 (BW25113_4504) | 259737..259958 | - | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| BW25113_RS01250 (BW25113_0247) | 259967..260443 | - | 477 | WP_000811693.1 | RadC family protein | - |
| BW25113_RS01255 (BW25113_0248) | 260459..260917 | - | 459 | WP_000211838.1 | antirestriction protein | - |
| BW25113_RS01260 (BW25113_0249) | 261015..261254 | - | 240 | WP_000194654.1 | DUF905 domain-containing protein | - |
| BW25113_RS01265 (BW25113_0250) | 261331..261798 | - | 468 | WP_001547765.1 | hypothetical protein | - |
| BW25113_RS25425 (BW25113_0251) | 261821..262264 | - | 444 | WP_000824223.1 | hypothetical protein | - |
| BW25113_RS25430 (BW25113_4627) | 262264..262491 | - | 228 | WP_001548158.1 | hypothetical protein | - |
| BW25113_RS25435 (BW25113_4628) | 262487..262678 | - | 192 | Protein_246 | DeoR family transcriptional regulator | - |
| BW25113_RS01280 (BW25113_0252) | 262895..263716 | - | 822 | WP_000197389.1 | DUF945 domain-containing protein | - |
| BW25113_RS01285 (BW25113_0253) | 263808..264671 | - | 864 | WP_001065553.1 | GTPase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T48669 WP_000854672.1 NZ_CP009273:c259380-259039 [Escherichia coli BW25113]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T48669 NZ_CP009273:c259380-259039 [Escherichia coli BW25113]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT48669 WP_000070395.1 NZ_CP009273:c259718-259401 [Escherichia coli BW25113]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT48669 NZ_CP009273:c259718-259401 [Escherichia coli BW25113]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|