Detailed information of TA system TA048665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 44998..45827
• Replicon: plasmid pOCN014
• Accession: NZ_CP009266
• Organism: Vibrio coralliilyticus strain OCN014

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: JV59_RS24770
• Protein ID: WP_040122443.1
• Coordinates: 44998..45561 (-)
• Length: 188 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7Y3YYU7
• Locus tag: JV59_RS24775
• Protein ID: WP_040122444.1
• Coordinates: 45558..45827 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JV59_RS24755	40059..41027	-	969	WP_040122440.1	hypothetical protein	-
JV59_RS26980	41159..41431	-	273	WP_144397619.1	hypothetical protein	-
JV59_RS24760	41496..43589	-	2094	WP_040122441.1	TonB-dependent siderophore receptor	-
JV59_RS24765	44513..44992	+	480	Protein_50	hypothetical protein	-
JV59_RS24770	44998..45561	-	564	WP_040122443.1	GNAT family N-acetyltransferase	Toxin
JV59_RS24775	45558..45827	-	270	WP_040122444.1	DUF1778 domain-containing protein	Antitoxin
JV59_RS24780	46034..46456	-	423	WP_040122445.1	GNAT family N-acetyltransferase	-
JV59_RS24785	46585..46989	-	405	WP_040122446.1	NUDIX domain-containing protein	-
JV59_RS24790	47436..47912	-	477	WP_144397632.1	DUF1311 domain-containing protein	-
JV59_RS24795	48079..48576	-	498	WP_040122447.1	GNAT family N-acetyltransferase	-
JV59_RS24800	48638..49006	-	369	WP_040122448.1	hypothetical protein	-
JV59_RS24805	49442..50296	+	855	WP_040122449.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..380781	380781

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-81)

Sequences

Toxin

Protein

• Length: 188 a.a.
• Molecular weight: 21000.48 Da
• Isoelectric Point: 9.6169

>T48665 WP_040122443.1 NZ_CP009266:c45561-44998 [Vibrio coralliilyticus]
VSWGNEFVELSKSMHDRSSFDCGESELNTFIKTLAAKHMQAGISRTMVLPSSQLLLNRKYAICAFYSVAPSSISRESLPA
KLAKTLPRYPIPVFLLAQLAVHKEFHGSGLGKISLIRALKYLWGVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNG
RIRMFLPMKTVEQLFSPKATNITKDDV

Nucleotide

• Length: 564 bp

>T48665 NZ_CP009266:c45561-44998 [Vibrio coralliilyticus]
GTGAGTTGGGGAAACGAGTTCGTTGAACTTAGTAAATCCATGCATGATCGCAGCTCATTTGATTGTGGTGAGTCAGAGTT
AAATACGTTTATTAAAACGCTAGCAGCAAAACACATGCAAGCAGGTATCAGCCGAACGATGGTTTTACCAAGCTCTCAGC
TACTGCTTAATCGAAAGTATGCTATTTGCGCTTTTTACAGTGTTGCTCCTAGTTCAATCAGTCGTGAATCTTTACCTGCT
AAGTTAGCGAAAACGTTACCTAGATATCCCATCCCCGTTTTTTTATTAGCTCAGCTAGCGGTGCATAAAGAATTTCATGG
CTCAGGATTAGGGAAAATCAGTTTAATTCGAGCGTTAAAGTATCTTTGGGGAGTGAATCACCACATGAGAGCTTATGCCA
TTGTTGTGGACTGCCTTACCGACTCCGCACAAGCATTCTATACTAAGTTTGGCTTTGAGGTTTTGTGTGAGCATAATGGG
CGTATACGTATGTTTTTACCAATGAAAACGGTAGAACAGCTTTTTAGTCCAAAGGCTACGAATATCACAAAAGATGATGT
CTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9839.34 Da
• Isoelectric Point: 4.5482

>AT48665 WP_040122444.1 NZ_CP009266:c45827-45558 [Vibrio coralliilyticus]
MATARLDIRLDEEIKAKAEKASALLGLKSLTEYVVRLMDEDATQVIEQHESIVVKESVFDEFMMACDKAKAPNQALLDAA
KLTNESGIK

Nucleotide

• Length: 270 bp

>AT48665 NZ_CP009266:c45827-45558 [Vibrio coralliilyticus]
ATGGCAACAGCACGTTTAGATATTCGCCTTGACGAAGAGATCAAAGCAAAAGCGGAGAAAGCATCGGCTTTACTGGGTCT
TAAAAGCCTTACTGAGTATGTAGTCAGACTTATGGATGAAGACGCCACTCAGGTAATTGAACAGCACGAAAGTATCGTAG
TCAAAGAGAGTGTCTTCGATGAATTCATGATGGCATGTGACAAAGCAAAGGCTCCCAATCAAGCTTTACTTGATGCAGCG
AAATTGACCAATGAGAGTGGCATCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7Y3YYU7