Detailed information of TA system TA048643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3756243..3756772
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: BCGR_RS17600
• Protein ID: WP_003417760.1
• Coordinates: 3756515..3756772 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: BCGR_RS17595
• Protein ID: WP_003417757.1
• Coordinates: 3756243..3756518 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS17560	3751274..3752572	-	1299	WP_011799343.1	PPE family protein	-
BCGR_RS22430	3753041..3754478	-	1438	Protein_3437	FAD-binding oxidoreductase	-
BCGR_RS17580	3754581..3754970	+	390	WP_010950876.1	DUF732 domain-containing protein	-
BCGR_RS17585	3754984..3755277	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
BCGR_RS17590	3755274..3756119	-	846	WP_010950877.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
BCGR_RS17595	3756243..3756518	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
BCGR_RS17600	3756515..3756772	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
BCGR_RS17605	3756814..3758004	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
BCGR_RS17610	3758121..3758489	+	369	WP_003417765.1	FHA domain-containing protein	-
BCGR_RS17615	3758486..3759037	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
BCGR_RS17620	3759044..3759625	-	582	WP_003417769.1	ATP/GTP-binding protein	-
BCGR_RS17625	3759606..3759974	-	369	WP_003417772.1	DUF742 domain-containing protein	-
BCGR_RS17630	3759952..3760344	-	393	WP_011799344.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T48643 WP_003417760.1 NZ_CP009243:3756515-3756772 [Mycobacterium tuberculosis variant bovis BCG]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T48643 NZ_CP009243:3756515-3756772 [Mycobacterium tuberculosis variant bovis BCG]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTAGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT48643 WP_003417757.1 NZ_CP009243:3756243-3756518 [Mycobacterium tuberculosis variant bovis BCG]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT48643 NZ_CP009243:3756243-3756518 [Mycobacterium tuberculosis variant bovis BCG]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: G0TI94