Detailed information of TA system TA048642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3694113..3694787
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: BCGR_RS17390
• Protein ID: WP_003417282.1
• Coordinates: 3694113..3694541 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: BCGR_RS17395
• Protein ID: WP_003417286.1
• Coordinates: 3694545..3694787 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS17365	3689935..3690336	-	402	WP_003417264.1	cytidine deaminase	-
BCGR_RS17370	3690477..3690911	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
BCGR_RS17375	3690908..3691342	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
BCGR_RS17380	3691471..3693243	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
BCGR_RS17385	3693243..3694034	+	792	WP_010950870.1	succinate dehydrogenase iron-sulfur subunit	-
BCGR_RS17390	3694113..3694541	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCGR_RS17395	3694545..3694787	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
BCGR_RS17400	3694909..3695523	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
BCGR_RS17405	3695520..3696185	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
BCGR_RS17410	3696186..3696719	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
BCGR_RS17415	3696716..3697090	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
BCGR_RS17420	3697187..3698251	-	1065	WP_003913037.1	GTP 3',8-cyclase MoaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T48642 WP_003417282.1 NZ_CP009243:c3694541-3694113 [Mycobacterium tuberculosis variant bovis BCG]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T48642 NZ_CP009243:c3694541-3694113 [Mycobacterium tuberculosis variant bovis BCG]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT48642 WP_003417286.1 NZ_CP009243:c3694787-3694545 [Mycobacterium tuberculosis variant bovis BCG]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT48642 NZ_CP009243:c3694787-3694545 [Mycobacterium tuberculosis variant bovis BCG]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59