Detailed information of TA system TA048640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3129902..3130589
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7U4FBY8
• Locus tag: BCGR_RS14850
• Protein ID: WP_011799298.1
• Coordinates: 3130146..3130589 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: BCGR_RS14845
• Protein ID: WP_003414620.1
• Coordinates: 3129902..3130159 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS14825	3125222..3126076	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
BCGR_RS14830	3126132..3127295	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
BCGR_RS14835	3127312..3128526	-	1215	WP_011799297.1	zinc metalloprotease Rip	-
BCGR_RS14840	3128534..3129775	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
BCGR_RS14845	3129902..3130159	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
BCGR_RS14850	3130146..3130589	+	444	WP_011799298.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCGR_RS14855	3130669..3131331	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
BCGR_RS14860	3131427..3131618	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BCGR_RS14865	3131950..3133698	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
BCGR_RS14870	3133794..3134375	+	582	WP_003414644.1	fasciclin domain-containing protein	-
BCGR_RS14875	3134475..3134741	+	267	WP_031657293.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16625.74 Da
• Isoelectric Point: 6.4890

>T48640 WP_011799298.1 NZ_CP009243:3130146-3130589 [Mycobacterium tuberculosis variant bovis BCG]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDTLLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T48640 NZ_CP009243:3130146-3130589 [Mycobacterium tuberculosis variant bovis BCG]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACACCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT48640 WP_003414620.1 NZ_CP009243:3129902-3130159 [Mycobacterium tuberculosis variant bovis BCG]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT48640 NZ_CP009243:3129902-3130159 [Mycobacterium tuberculosis variant bovis BCG]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBY8

Antitoxin

• AlphaFold DB: G0TFH0