Detailed information of TA system TA048636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3056393..3056963
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: BCGR_RS14465
• Protein ID: WP_003414166.1
• Coordinates: 3056393..3056749 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: BCGR_RS14470
• Protein ID: WP_003901465.1
• Coordinates: 3056733..3056963 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS14445	3051845..3053533	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
BCGR_RS14450	3053537..3053863	-	327	WP_003414157.1	hypothetical protein	-
BCGR_RS14455	3054036..3054623	+	588	WP_003914429.1	DUF3558 family protein	-
BCGR_RS14460	3054642..3056291	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
BCGR_RS14465	3056393..3056749	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
BCGR_RS14470	3056733..3056963	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
BCGR_RS14475	3057006..3058049	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
BCGR_RS21880	3058240..3058515	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
BCGR_RS21885	3058691..3058945	-	255	WP_003917684.1	hypothetical protein	-
BCGR_RS14490	3059093..3059497	+	405	WP_003414181.1	hypothetical protein	-
BCGR_RS14495	3059494..3059685	+	192	WP_003414184.1	hypothetical protein	-
BCGR_RS14500	3059749..3061038	+	1290	Protein_2828	transposase family protein	-
BCGR_RS14505	3061272..3061529	+	258	WP_003899489.1	hypothetical protein	-
BCGR_RS14510	3061634..3061945	+	312	WP_010950777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T48636 WP_003414166.1 NZ_CP009243:c3056749-3056393 [Mycobacterium tuberculosis variant bovis BCG]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T48636 NZ_CP009243:c3056749-3056393 [Mycobacterium tuberculosis variant bovis BCG]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT48636 WP_003901465.1 NZ_CP009243:c3056963-3056733 [Mycobacterium tuberculosis variant bovis BCG]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT48636 NZ_CP009243:c3056963-3056733 [Mycobacterium tuberculosis variant bovis BCG]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3