Detailed information of TA system TA048634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3016397..3017076
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: BCGR_RS14235
• Protein ID: WP_003414059.1
• Coordinates: 3016397..3016813 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: BCGR_RS14240
• Protein ID: WP_003414061.1
• Coordinates: 3016810..3017076 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS14215	3012449..3013351	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BCGR_RS14220	3013420..3014172	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
BCGR_RS14225	3014416..3014691	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
BCGR_RS14230	3014688..3016310	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
BCGR_RS14235	3016397..3016813	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
BCGR_RS14240	3016810..3017076	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BCGR_RS14245	3017102..3017497	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
BCGR_RS14250	3017494..3017763	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
BCGR_RS14255	3017773..3018867	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
BCGR_RS14260	3018864..3019283	-	420	Protein_2781	winged helix-turn-helix domain-containing protein	-
BCGR_RS14265	3019357..3019836	-	480	WP_003414073.1	dihydrofolate reductase	-
BCGR_RS14270	3019907..3020707	-	801	WP_003911953.1	thymidylate synthase	-
BCGR_RS14275	3020863..3021600	+	738	WP_011799288.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T48634 WP_003414059.1 NZ_CP009243:c3016813-3016397 [Mycobacterium tuberculosis variant bovis BCG]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T48634 NZ_CP009243:c3016813-3016397 [Mycobacterium tuberculosis variant bovis BCG]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT48634 WP_003414061.1 NZ_CP009243:c3017076-3016810 [Mycobacterium tuberculosis variant bovis BCG]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT48634 NZ_CP009243:c3017076-3016810 [Mycobacterium tuberculosis variant bovis BCG]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64