48632

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2826957..2827594 Replicon chromosome
Accession NZ_CP009243
Organism Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag BCGR_RS13245 Protein ID WP_003413180.1
Coordinates 2826957..2827352 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag BCGR_RS13250 Protein ID WP_003413183.1
Coordinates 2827349..2827594 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCGR_RS13205 2823042..2823701 + 660 WP_003900846.1 LppA family lipoprotein -
BCGR_RS13210 2823698..2824357 + 660 WP_003900847.1 hypothetical protein -
BCGR_RS13215 2824354..2825016 + 663 WP_003900848.1 hypothetical protein -
BCGR_RS13220 2825013..2825291 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
BCGR_RS13225 2825384..2825797 + 414 WP_003413164.1 PIN domain nuclease -
BCGR_RS13230 2825836..2826093 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
BCGR_RS13235 2826090..2826467 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
BCGR_RS13240 2826483..2826857 - 375 WP_003413177.1 hypothetical protein -
BCGR_RS13245 2826957..2827352 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
BCGR_RS13250 2827349..2827594 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
BCGR_RS13255 2828005..2828424 - 420 WP_003413190.1 A24 family peptidase -
BCGR_RS13260 2828436..2829263 - 828 WP_010950736.1 shikimate dehydrogenase -
BCGR_RS13265 2829241..2830494 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BCGR_RS13270 2830487..2830999 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T48632 WP_003413180.1 NZ_CP009243:c2827352-2826957 [Mycobacterium tuberculosis variant bovis BCG]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T48632 NZ_CP009243:c2827352-2826957 [Mycobacterium tuberculosis variant bovis BCG]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT48632 WP_003413183.1 NZ_CP009243:c2827594-2827349 [Mycobacterium tuberculosis variant bovis BCG]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT48632 NZ_CP009243:c2827594-2827349 [Mycobacterium tuberculosis variant bovis BCG]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References