Detailed information of TA system TA048623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2229481..2230107
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: BCGR_RS10390
• Protein ID: WP_003410075.1
• Coordinates: 2229709..2230107 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BCGR_RS10385
• Protein ID: WP_019283586.1
• Coordinates: 2229481..2229708 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS10375	2227520..2227864	-	345	WP_003410065.1	ferredoxin family protein	-
BCGR_RS10380	2228053..2229222	-	1170	WP_003899126.1	ATP-binding protein	-
BCGR_RS10385	2229481..2229708	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BCGR_RS10390	2229709..2230107	+	399	WP_003410075.1	PIN domain nuclease	Toxin
BCGR_RS10395	2230290..2230721	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
BCGR_RS10400	2230822..2231256	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
BCGR_RS21015	2231693..2231872	-	180	Protein_2033	hypothetical protein	-
BCGR_RS10405	2231933..2232577	+	645	WP_078396697.1	transposase	-
BCGR_RS10410	2232531..2233121	+	591	WP_003899131.1	IS110 family transposase	-
BCGR_RS10415	2233249..2234505	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T48623 WP_003410075.1 NZ_CP009243:2229709-2230107 [Mycobacterium tuberculosis variant bovis BCG]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T48623 NZ_CP009243:2229709-2230107 [Mycobacterium tuberculosis variant bovis BCG]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT48623 WP_019283586.1 NZ_CP009243:2229481-2229708 [Mycobacterium tuberculosis variant bovis BCG]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT48623 NZ_CP009243:2229481-2229708 [Mycobacterium tuberculosis variant bovis BCG]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG