Detailed information of TA system TA048620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2196849..2197537
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0A653
• Locus tag: BCGR_RS10225
• Protein ID: WP_003409958.1
• Coordinates: 2196849..2197268 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ28
• Locus tag: BCGR_RS10230
• Protein ID: WP_003409968.1
• Coordinates: 2197277..2197537 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS10200	2191855..2192160	+	306	Protein_1991	ABC transporter permease	-
BCGR_RS10205	2192344..2193192	+	849	WP_003409942.1	class I SAM-dependent methyltransferase	-
BCGR_RS10210	2193155..2194600	-	1446	WP_003409946.1	APC family permease	-
BCGR_RS10215	2194779..2195465	-	687	WP_003409954.1	immunoprotective protein Mpt64	-
BCGR_RS10220	2195656..2196624	-	969	WP_003409956.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
BCGR_RS10225	2196849..2197268	-	420	WP_003409958.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCGR_RS10230	2197277..2197537	-	261	WP_003409968.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BCGR_RS10235	2197680..2199356	+	1677	WP_010950639.1	PecA family PE domain-processing aspartic protease	-
BCGR_RS10240	2199344..2199997	-	654	WP_003409976.1	cutinase Cfp21	-
BCGR_RS10245	2200112..2200342	+	231	WP_003409980.1	DUF167 domain-containing protein	-
BCGR_RS10250	2200427..2201338	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
BCGR_RS10255	2201447..2202046	+	600	WP_003409989.1	L-lysine exporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14724.89 Da
• Isoelectric Point: 7.9535

>T48620 WP_003409958.1 NZ_CP009243:c2197268-2196849 [Mycobacterium tuberculosis variant bovis BCG]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Nucleotide

• Length: 420 bp

>T48620 NZ_CP009243:c2197268-2196849 [Mycobacterium tuberculosis variant bovis BCG]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9528.88 Da
• Isoelectric Point: 5.1007

>AT48620 WP_003409968.1 NZ_CP009243:c2197537-2197277 [Mycobacterium tuberculosis variant bovis BCG]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Nucleotide

• Length: 261 bp

>AT48620 NZ_CP009243:c2197537-2197277 [Mycobacterium tuberculosis variant bovis BCG]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Toxin

• AlphaFold DB: A0A829C4H9

Antitoxin

• AlphaFold DB: A0A829C483