Detailed information of TA system TA048617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2178798..2179501
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BCGR_RS10075
• Protein ID: WP_003409778.1
• Coordinates: 2178798..2179127 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BCGR_RS20990
• Protein ID: WP_003409780.1
• Coordinates: 2179124..2179501 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS10055	2175181..2176251	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
BCGR_RS10060	2176248..2176763	+	516	WP_003409718.1	flavin reductase family protein	-
BCGR_RS10065	2176760..2177821	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BCGR_RS10070	2177818..2178588	+	771	WP_003409775.1	SDR family oxidoreductase	-
BCGR_RS10075	2178798..2179127	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BCGR_RS20990	2179124..2179501	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BCGR_RS10085	2179498..2180088	-	591	WP_003910477.1	SEC-C domain-containing protein	-
BCGR_RS10090	2180143..2181507	+	1365	WP_011799233.1	HNH endonuclease	-
BCGR_RS10095	2181662..2182114	-	453	WP_010950636.1	lipoprotein	-
BCGR_RS10100	2182178..2182579	+	402	WP_003409869.1	hypothetical protein	-
BCGR_RS10105	2182572..2182754	-	183	WP_003409870.1	hypothetical protein	-
BCGR_RS10110	2182868..2183218	-	351	WP_003409871.1	hypothetical protein	-
BCGR_RS10115	2183229..2184131	-	903	WP_003409874.1	hypothetical protein	-
BCGR_RS10120	2184152..2184343	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T48617 WP_003409778.1 NZ_CP009243:c2179127-2178798 [Mycobacterium tuberculosis variant bovis BCG]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T48617 NZ_CP009243:c2179127-2178798 [Mycobacterium tuberculosis variant bovis BCG]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT48617 WP_003409780.1 NZ_CP009243:c2179501-2179124 [Mycobacterium tuberculosis variant bovis BCG]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT48617 NZ_CP009243:c2179501-2179124 [Mycobacterium tuberculosis variant bovis BCG]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8