Detailed information of TA system TA048609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1386882..1387570
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: BCGR_RS06600
• Protein ID: WP_003406304.1
• Coordinates: 1387139..1387570 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: BCGR_RS06595
• Protein ID: WP_003406302.1
• Coordinates: 1386882..1387142 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS06575	1382459..1383283	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
BCGR_RS06580	1383288..1384469	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
BCGR_RS06585	1384546..1385646	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
BCGR_RS06590	1385817..1386806	+	990	WP_003406301.1	malate dehydrogenase	-
BCGR_RS06595	1386882..1387142	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
BCGR_RS06600	1387139..1387570	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCGR_RS06605	1387593..1389281	-	1689	WP_003910308.1	PE family protein	-
BCGR_RS06610	1389461..1390321	+	861	WP_003406306.1	hypothetical protein	-
BCGR_RS06615	1390402..1391232	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
BCGR_RS06620	1391289..1391582	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
BCGR_RS06625	1391579..1391848	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T48609 WP_003406304.1 NZ_CP009243:1387139-1387570 [Mycobacterium tuberculosis variant bovis BCG]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T48609 NZ_CP009243:1387139-1387570 [Mycobacterium tuberculosis variant bovis BCG]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT48609 WP_003406302.1 NZ_CP009243:1386882-1387142 [Mycobacterium tuberculosis variant bovis BCG]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT48609 NZ_CP009243:1386882-1387142 [Mycobacterium tuberculosis variant bovis BCG]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6