Detailed information of TA system TA048608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1242166..1242734
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: BCGR_RS05930
• Protein ID: WP_003405865.1
• Coordinates: 1242360..1242734 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: BCGR_RS05925
• Protein ID: WP_003405863.1
• Coordinates: 1242166..1242363 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS05905	1238207..1238845	-	639	WP_011799158.1	lipid droplet-associated protein	-
BCGR_RS05910	1238935..1239942	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BCGR_RS05915	1239959..1240942	-	984	WP_003898731.1	hypothetical protein	-
BCGR_RS05920	1241005..1242078	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
BCGR_RS05925	1242166..1242363	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BCGR_RS05930	1242360..1242734	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCGR_RS05935	1242937..1243635	+	699	WP_010950494.1	hypothetical protein	-
BCGR_RS05940	1243753..1243938	+	186	WP_003901093.1	hypothetical protein	-
BCGR_RS05945	1243865..1244140	-	276	WP_003405867.1	hypothetical protein	-
BCGR_RS05950	1244383..1244706	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
BCGR_RS05955	1244721..1245431	-	711	WP_003911399.1	hypothetical protein	-
BCGR_RS22280	1245453..1245662	+	210	WP_003911400.1	hypothetical protein	-
BCGR_RS05965	1245614..1246321	-	708	Protein_1159	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T48608 WP_003405865.1 NZ_CP009243:1242360-1242734 [Mycobacterium tuberculosis variant bovis BCG]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T48608 NZ_CP009243:1242360-1242734 [Mycobacterium tuberculosis variant bovis BCG]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT48608 WP_003405863.1 NZ_CP009243:1242166-1242363 [Mycobacterium tuberculosis variant bovis BCG]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT48608 NZ_CP009243:1242166-1242363 [Mycobacterium tuberculosis variant bovis BCG]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07