Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1233410..1234041 Replicon chromosome
Accession NZ_CP009243
Organism Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag BCGR_RS05870 Protein ID WP_003405820.1
Coordinates 1233410..1233721 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag BCGR_RS05875 Protein ID WP_003405836.1
Coordinates 1233721..1234041 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCGR_RS05850 1228892..1230316 - 1425 WP_003405805.1 class II fumarate hydratase -
BCGR_RS05855 1230347..1231435 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
BCGR_RS05860 1231491..1232135 + 645 WP_174813088.1 DUF4245 domain-containing protein -
BCGR_RS05865 1232142..1233298 - 1157 Protein_1140 AI-2E family transporter -
BCGR_RS05870 1233410..1233721 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
BCGR_RS05875 1233721..1234041 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
BCGR_RS20725 1234051..1235576 + 1526 Protein_1143 carboxylesterase/lipase family protein -
BCGR_RS05890 1235594..1236706 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
BCGR_RS05895 1236716..1236973 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
BCGR_RS05900 1236963..1238210 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
BCGR_RS05905 1238207..1238845 - 639 WP_011799158.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T48607 WP_003405820.1 NZ_CP009243:c1233721-1233410 [Mycobacterium tuberculosis variant bovis BCG]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T48607 NZ_CP009243:c1233721-1233410 [Mycobacterium tuberculosis variant bovis BCG]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT48607 WP_003405836.1 NZ_CP009243:c1234041-1233721 [Mycobacterium tuberculosis variant bovis BCG]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT48607 NZ_CP009243:c1234041-1233721 [Mycobacterium tuberculosis variant bovis BCG]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References