Detailed information of TA system TA048604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1016870..1017489
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: BCGR_RS04805
• Protein ID: WP_003404726.1
• Coordinates: 1017055..1017489 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: BCGR_RS04800
• Protein ID: WP_003404724.1
• Coordinates: 1016870..1017049 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS04790	1012343..1013923	+	1581	WP_011799139.1	serine hydrolase	-
BCGR_RS04795	1013920..1016313	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
BCGR_RS22245	1016586..1016744	+	159	WP_003404720.1	hypothetical protein	-
BCGR_RS04800	1016870..1017049	+	180	WP_003404724.1	antitoxin	Antitoxin
BCGR_RS04805	1017055..1017489	+	435	WP_003404726.1	SRPBCC family protein	Toxin
BCGR_RS04810	1017587..1018360	+	774	WP_003404735.1	VOC family protein	-
BCGR_RS04815	1018425..1018874	+	450	WP_003404738.1	hypothetical protein	-
BCGR_RS04820	1019009..1019239	-	231	WP_003898642.1	hypothetical protein	-
BCGR_RS04825	1019406..1020914	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
BCGR_RS04830	1020916..1022154	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T48604 WP_003404726.1 NZ_CP009243:1017055-1017489 [Mycobacterium tuberculosis variant bovis BCG]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T48604 NZ_CP009243:1017055-1017489 [Mycobacterium tuberculosis variant bovis BCG]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT48604 WP_003404724.1 NZ_CP009243:1016870-1017049 [Mycobacterium tuberculosis variant bovis BCG]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT48604 NZ_CP009243:1016870-1017049 [Mycobacterium tuberculosis variant bovis BCG]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5