Detailed information of TA system TA048602

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 757102..757790
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: BCGR_RS03480
• Protein ID: WP_003403386.1
• Coordinates: 757102..757539 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O06777
• Locus tag: BCGR_RS03485
• Protein ID: WP_003911263.1
• Coordinates: 757536..757790 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS03450	753353..754363	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
BCGR_RS03455	754751..755134	-	384	WP_003403365.1	ribonuclease VapC6	-
BCGR_RS03460	755229..755384	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
BCGR_RS03465	755460..756176	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
BCGR_RS03470	756452..756760	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
BCGR_RS03475	756747..756992	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
BCGR_RS03480	757102..757539	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCGR_RS03485	757536..757790	-	255	WP_003911263.1	antitoxin VapB7	Antitoxin
BCGR_RS03490	757904..760267	+	2364	WP_003403397.1	arylsulfatase AtsD	-
BCGR_RS03495	760330..760656	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BCGR_RS03500	760568..760906	+	339	WP_003403405.1	PIN domain-containing protein	-
BCGR_RS03505	760903..761076	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T48602 WP_003403386.1 NZ_CP009243:c757539-757102 [Mycobacterium tuberculosis variant bovis BCG]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T48602 NZ_CP009243:c757539-757102 [Mycobacterium tuberculosis variant bovis BCG]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9450.99 Da
• Isoelectric Point: 11.7073

>AT48602 WP_003911263.1 NZ_CP009243:c757790-757536 [Mycobacterium tuberculosis variant bovis BCG]
MSMRLAHRLQILLDDECHRRITAVARERGVPVATVVREAIDRGLVSPAGRRKSAGRRLLDAADMSVPEPRELKQELEALR
ARRG

Nucleotide

• Length: 255 bp

>AT48602 NZ_CP009243:c757790-757536 [Mycobacterium tuberculosis variant bovis BCG]
ATGAGCATGCGTCTGGCGCATCGGCTCCAGATCCTGCTCGACGACGAGTGCCACCGCCGCATCACCGCGGTGGCGCGCGA
GCGAGGCGTCCCGGTCGCAACCGTCGTGCGTGAGGCCATTGATCGAGGCCTCGTCAGCCCCGCCGGTCGCCGCAAGTCCG
CGGGCAGGCGCCTGCTCGACGCCGCCGACATGTCCGTTCCCGAGCCGCGGGAGCTGAAGCAGGAACTCGAGGCCCTCCGG
GCGCGCCGCGGATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: A0A806JQG1