Detailed information of TA system TA048595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 704487..705130
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: BCGR_RS03180
• Protein ID: WP_003403187.1
• Coordinates: 704729..705130 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: BCGR_RS03175
• Protein ID: WP_003403184.1
• Coordinates: 704487..704732 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS03140	699767..700297	-	531	WP_003917320.1	two component system sensor kinase HK2	-
BCGR_RS03145	700281..700976	-	696	WP_197044064.1	response regulator transcription factor	-
BCGR_RS03150	701099..701410	+	312	WP_003403164.1	hypothetical protein	-
BCGR_RS03155	701482..702432	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
BCGR_RS03160	702673..703257	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
BCGR_RS03165	703259..703972	+	714	Protein_626	IS607 family element transposase accessory protein TnpB	-
BCGR_RS03170	703972..704442	+	471	WP_003898523.1	hypothetical protein	-
BCGR_RS03175	704487..704732	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BCGR_RS03180	704729..705130	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCGR_RS21720	705518..705685	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
BCGR_RS21725	705718..705915	-	198	WP_003403191.1	hypothetical protein	-
BCGR_RS03195	705995..707152	-	1158	WP_003403193.1	hypothetical protein	-
BCGR_RS03200	707204..707425	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
BCGR_RS03205	707567..708172	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T48595 WP_003403187.1 NZ_CP009243:704729-705130 [Mycobacterium tuberculosis variant bovis BCG]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T48595 NZ_CP009243:704729-705130 [Mycobacterium tuberculosis variant bovis BCG]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT48595 WP_003403184.1 NZ_CP009243:704487-704732 [Mycobacterium tuberculosis variant bovis BCG]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT48595 NZ_CP009243:704487-704732 [Mycobacterium tuberculosis variant bovis BCG]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91