Detailed information of TA system TA048593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 696082..696728
• Replicon: chromosome
• Accession: NZ_CP009243
• Organism: Mycobacterium tuberculosis variant bovis BCG strain Russia 368

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O07783
• Locus tag: BCGR_RS03110
• Protein ID: WP_003403122.1
• Coordinates: 696082..696474 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ86
• Locus tag: BCGR_RS03115
• Protein ID: WP_003403125.1
• Coordinates: 696471..696728 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCGR_RS03095	691742..693270	+	1529	Protein_612	virulence factor Mce family protein	-
BCGR_RS03100	693333..694475	+	1143	WP_003911253.1	virulence factor Mce family protein	-
BCGR_RS03105	694480..696030	+	1551	WP_003403119.1	MCE family protein	-
BCGR_RS03110	696082..696474	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	Toxin
BCGR_RS03115	696471..696728	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	Antitoxin
BCGR_RS03120	696911..698146	-	1236	WP_003403128.1	ATP-binding protein	-
BCGR_RS03125	698397..698810	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	-
BCGR_RS03130	698807..699043	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
BCGR_RS03135	699147..699653	-	507	WP_003900973.1	two component system sensor kinase HK1	-
BCGR_RS03140	699767..700297	-	531	WP_003917320.1	two component system sensor kinase HK2	-
BCGR_RS03145	700281..700976	-	696	WP_197044064.1	response regulator transcription factor	-
BCGR_RS03150	701099..701410	+	312	WP_003403164.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14080.08 Da
• Isoelectric Point: 4.6558

>T48593 WP_003403122.1 NZ_CP009243:c696474-696082 [Mycobacterium tuberculosis variant bovis BCG]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Nucleotide

• Length: 393 bp

>T48593 NZ_CP009243:c696474-696082 [Mycobacterium tuberculosis variant bovis BCG]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9681.06 Da
• Isoelectric Point: 7.0175

>AT48593 WP_003403125.1 NZ_CP009243:c696728-696471 [Mycobacterium tuberculosis variant bovis BCG]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Nucleotide

• Length: 258 bp

>AT48593 NZ_CP009243:c696728-696471 [Mycobacterium tuberculosis variant bovis BCG]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4F8V4

Antitoxin

• AlphaFold DB: G0TQ86