Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 774285..774865 Replicon chromosome
Accession NZ_CP009237
Organism Glaesserella parasuis strain KL0318

Toxin (Protein)


Gene name mazF Uniprot ID A0A143CFE3
Locus tag JT17_RS03740 Protein ID WP_005710599.1
Coordinates 774521..774865 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U4SAI4
Locus tag JT17_RS03735 Protein ID WP_005710601.1
Coordinates 774285..774524 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT17_RS03710 769989..770681 - 693 WP_005710610.1 tellurite resistance TerB family protein -
JT17_RS03715 770743..771591 - 849 WP_021110479.1 ABC transporter permease subunit -
JT17_RS03720 771588..772373 - 786 WP_015940139.1 ABC transporter permease subunit -
JT17_RS03725 772363..773112 - 750 WP_044009125.1 phosphonate ABC transporter ATP-binding protein -
JT17_RS03730 773224..774099 - 876 WP_021119465.1 putative selenate ABC transporter substrate-binding protein -
JT17_RS03735 774285..774524 + 240 WP_005710601.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JT17_RS03740 774521..774865 + 345 WP_005710599.1 endoribonuclease MazF Toxin
JT17_RS03745 774871..775539 + 669 WP_005710597.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
JT17_RS03750 775552..776943 + 1392 WP_005710595.1 MATE family efflux transporter -
JT17_RS03755 777016..779121 - 2106 WP_044009126.1 glycoside hydrolase family 2 -
JT17_RS03760 779327..779623 - 297 WP_005710591.1 DUF5374 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12830.81 Da        Isoelectric Point: 7.9277

>T48580 WP_005710599.1 NZ_CP009237:774521-774865 [Glaesserella parasuis]
MTTQEYIPDVGDIIWLDFDPQAGHEQAGHRPALVLTPTIYNRQTGLLICCPLTTKVKGYPFEVNIAGTPQNVVLSDQIKS
LDWRIRHAKFKGKINHHQLSEVKAKIATLLQISE

Download         Length: 345 bp

>T48580 NZ_CP009237:774521-774865 [Glaesserella parasuis]
ATGACTACTCAAGAGTATATCCCTGACGTTGGTGATATTATTTGGCTTGATTTTGATCCGCAAGCAGGACATGAACAAGC
TGGCCATCGTCCAGCATTAGTGTTAACGCCCACGATTTATAATCGTCAAACAGGGTTGTTAATTTGCTGCCCGCTCACAA
CAAAAGTAAAAGGCTATCCTTTCGAAGTCAATATTGCAGGTACCCCCCAAAATGTTGTGCTTTCCGATCAAATTAAAAGC
CTTGACTGGCGTATTCGTCACGCTAAATTTAAAGGTAAAATTAACCATCATCAGTTAAGCGAAGTAAAAGCTAAAATTGC
GACTTTGCTACAAATCAGTGAATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8782.21 Da        Isoelectric Point: 5.0351

>AT48580 WP_005710601.1 NZ_CP009237:774285-774524 [Glaesserella parasuis]
MQVAVKKWGNSVGIRIPALLSNSLKLQAESLVDIQEVDGRLIIEPIKPKYNLEQLLSGITEDNLHNEVDFGKPVGKEML

Download         Length: 240 bp

>AT48580 NZ_CP009237:774285-774524 [Glaesserella parasuis]
ATGCAAGTGGCTGTCAAAAAATGGGGAAATAGCGTAGGCATTCGCATTCCTGCTTTATTATCTAATTCATTGAAATTACA
GGCAGAGAGTTTAGTCGATATTCAAGAAGTTGATGGTCGCCTAATTATTGAGCCGATTAAACCTAAATATAATTTAGAGC
AACTTCTTTCAGGTATTACTGAAGATAATTTGCACAATGAAGTCGATTTTGGCAAGCCTGTTGGCAAGGAAATGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A143CFE3

Antitoxin

Source ID Structure
AlphaFold DB U4SAI4

References