Detailed information of TA system TA048575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3015859..3016514
• Replicon: chromosome
• Accession: NZ_CP009235
• Organism: Burkholderia pseudomallei MSHR62

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0F6LEW6
• Locus tag: Y028_RS26445
• Protein ID: WP_006027262.1
• Coordinates: 3016218..3016514 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: Y028_RS26440
• Protein ID: WP_004202809.1
• Coordinates: 3015859..3016215 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y028_RS26420	3010994..3012007	-	1014	WP_041224479.1	phage major capsid protein, P2 family	-
Y028_RS26425	3012041..3012850	-	810	WP_041189666.1	GPO family capsid scaffolding protein	-
Y028_RS26430	3012994..3014763	+	1770	WP_041224545.1	terminase ATPase subunit family protein	-
Y028_RS26435	3014760..3015815	+	1056	WP_038726677.1	phage portal protein	-
Y028_RS26440	3015859..3016215	-	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
Y028_RS26445	3016218..3016514	-	297	WP_006027262.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Y028_RS39520	3017251..3017868	+	618	WP_144403433.1	hypothetical protein	-
Y028_RS37660	3018101..3018229	-	129	WP_038781962.1	ribbon-helix-helix protein, CopG family	-
Y028_RS26455	3018454..3019110	-	657	WP_038781956.1	AAA family ATPase	-
Y028_RS26460	3019200..3019406	-	207	WP_004532731.1	ogr/Delta-like zinc finger family protein	-
Y028_RS26465	3019403..3019669	-	267	WP_038789831.1	ogr/Delta-like zinc finger family protein	-
Y028_RS26470	3019685..3021478	-	1794	WP_041224481.1	replication endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2986079..3025938	39859

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10844.65 Da
• Isoelectric Point: 10.1723

>T48575 WP_006027262.1 NZ_CP009235:c3016514-3016218 [Burkholderia pseudomallei MSHR62]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED

Nucleotide

• Length: 297 bp

>T48575 NZ_CP009235:c3016514-3016218 [Burkholderia pseudomallei MSHR62]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTATTTCGTGCGGCGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT48575 WP_004202809.1 NZ_CP009235:c3016215-3015859 [Burkholderia pseudomallei MSHR62]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT48575 NZ_CP009235:c3016215-3015859 [Burkholderia pseudomallei MSHR62]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGGAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A0F6LEW6

Antitoxin

• AlphaFold DB: A0A3N4G1D3