Detailed information of TA system TA048572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 763842..764283
• Replicon: chromosome
• Accession: NZ_CP009234
• Organism: Burkholderia pseudomallei MSHR62

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: Y028_RS31075
• Protein ID: WP_017881988.1
• Coordinates: 763842..764015 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: Y028_RS03210
• Protein ID: WP_004525420.1
• Coordinates: 763999..764283 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y028_RS40620	759720..760148	-	429	Protein_626	DDE-type integrase/transposase/recombinase	-
Y028_RS40625	760146..760919	-	774	Protein_627	prohibitin family protein	-
Y028_RS03175	760916..761065	-	150	WP_004537646.1	bacteriophage protein Gp44	-
Y028_RS32070	761314..761967	+	654	WP_038734757.1	hypothetical protein	-
Y028_RS40055	762153..762452	-	300	WP_161788725.1	hypothetical protein	-
Y028_RS03190	762419..762640	-	222	WP_029248167.1	hypothetical protein	-
Y028_RS03205	762917..763192	-	276	WP_038726742.1	hypothetical protein	-
Y028_RS31075	763842..764015	+	174	WP_017881988.1	BrnT family toxin	Toxin
Y028_RS03210	763999..764283	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
Y028_RS32085	764930..765313	+	384	Protein_635	DDE-type integrase/transposase/recombinase	-
Y028_RS32090	765276..765704	+	429	Protein_636	IS110 family transposase	-
Y028_RS03225	765931..766047	-	117	Protein_637	IS3 family transposase	-
Y028_RS03230	766313..767773	+	1461	WP_023358159.1	metallophosphoesterase	-
Y028_RS03235	767932..769194	+	1263	WP_004537187.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	757409..771415	14006

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6663.54 Da
• Isoelectric Point: 7.9950

>T48572 WP_017881988.1 NZ_CP009234:763842-764015 [Burkholderia pseudomallei MSHR62]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREAKSYVEQA

Nucleotide

• Length: 174 bp

>T48572 NZ_CP009234:763842-764015 [Burkholderia pseudomallei MSHR62]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGCCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT48572 WP_004525420.1 NZ_CP009234:763999-764283 [Burkholderia pseudomallei MSHR62]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT48572 NZ_CP009234:763999-764283 [Burkholderia pseudomallei MSHR62]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAACCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8