Detailed information of TA system TA048559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1501536..1502073
• Replicon: chromosome
• Accession: NZ_CP009210
• Organism: Burkholderia pseudomallei strain BDP

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: JE55_RS24685
• Protein ID: WP_009923450.1
• Coordinates: 1501536..1501805 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: JE55_RS24690
• Protein ID: WP_004525420.1
• Coordinates: 1501789..1502073 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE55_RS42365	1497513..1497941	-	429	Protein_1270	DDE-type integrase/transposase/recombinase	-
JE55_RS42370	1497939..1498712	-	774	Protein_1271	prohibitin family protein	-
JE55_RS24645	1498709..1498858	-	150	WP_004542626.1	bacteriophage protein Gp44	-
JE55_RS24650	1499114..1499695	+	582	WP_004542548.1	hypothetical protein	-
JE55_RS41920	1499926..1500144	-	219	WP_004552392.1	hypothetical protein	-
JE55_RS24660	1500192..1500413	-	222	WP_004525418.1	hypothetical protein	-
JE55_RS24675	1500690..1500965	-	276	WP_004552388.1	bacteriophage protein Gp49	-
JE55_RS24685	1501536..1501805	+	270	WP_009923450.1	BrnT family toxin	Toxin
JE55_RS24690	1501789..1502073	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
JE55_RS37795	1502720..1503101	+	382	Protein_1279	DDE-type integrase/transposase/recombinase	-
JE55_RS37800	1503064..1503491	+	428	Protein_1280	IS110 family transposase	-
JE55_RS24700	1503718..1503834	-	117	Protein_1281	IS3 family transposase	-
JE55_RS24705	1504081..1505541	+	1461	WP_020850157.1	metallophosphoesterase	-
JE55_RS24710	1505700..1506962	+	1263	WP_020850375.1	cytochrome c family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1495202..1509184	13982

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10156.52 Da
• Isoelectric Point: 7.1633

>T48559 WP_009923450.1 NZ_CP009210:1501536-1501805 [Burkholderia pseudomallei]
MDITFDPTKNKTNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T48559 NZ_CP009210:1501536-1501805 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACAAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT48559 WP_004525420.1 NZ_CP009210:1501789-1502073 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT48559 NZ_CP009210:1501789-1502073 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8