Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2137765..2138384 Replicon chromosome
Accession NZ_CP009208
Organism Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag VK055_RS10560 Protein ID WP_002892050.1
Coordinates 2138166..2138384 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag VK055_RS10555 Protein ID WP_002892066.1
Coordinates 2137765..2138139 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VK055_RS10545 2132917..2134110 + 1194 WP_038432021.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
VK055_RS10550 2134133..2137279 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
VK055_RS10555 2137765..2138139 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
VK055_RS10560 2138166..2138384 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
VK055_RS10565 2138543..2139109 + 567 WP_002892030.1 maltose O-acetyltransferase -
VK055_RS28875 2139081..2139221 - 141 WP_004147370.1 hypothetical protein -
VK055_RS10575 2139242..2139712 + 471 WP_002892026.1 YlaC family protein -
VK055_RS10580 2139687..2141138 - 1452 WP_038432022.1 PLP-dependent aminotransferase family protein -
VK055_RS10585 2141239..2141937 + 699 WP_004177238.1 GNAT family N-acetyltransferase -
VK055_RS10590 2141934..2142074 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
VK055_RS10595 2142074..2142337 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T48548 WP_002892050.1 NZ_CP009208:2138166-2138384 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T48548 NZ_CP009208:2138166-2138384 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT48548 WP_002892066.1 NZ_CP009208:2137765-2138139 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT48548 NZ_CP009208:2137765-2138139 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGCCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References