Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1829652..1830280 Replicon chromosome
Accession NZ_CP009170
Organism Thermoanaerobacter kivui strain LKT-1

Toxin (Protein)


Gene name mazF Uniprot ID B0KD57
Locus tag TKV_RS09185 Protein ID WP_003867472.1
Coordinates 1829652..1830002 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M8DD10
Locus tag TKV_RS09190 Protein ID WP_003870023.1
Coordinates 1830005..1830280 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TKV_RS09165 1825142..1825879 - 738 WP_049685679.1 WecB/TagA/CpsF family glycosyltransferase -
TKV_RS09170 1825882..1826973 - 1092 WP_049685680.1 polysaccharide pyruvyl transferase CsaB -
TKV_RS09175 1826975..1828987 - 2013 WP_049685681.1 hypothetical protein -
TKV_RS09180 1829102..1829554 - 453 WP_049685682.1 hypothetical protein -
TKV_RS09185 1829652..1830002 - 351 WP_003867472.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
TKV_RS09190 1830005..1830280 - 276 WP_003870023.1 ribbon-helix-helix protein, CopG family Antitoxin
TKV_RS09195 1830370..1831536 - 1167 WP_049686269.1 alanine racemase -
TKV_RS09200 1831556..1832587 - 1032 WP_049685683.1 outer membrane lipoprotein carrier protein LolA -
TKV_RS09205 1832575..1834107 - 1533 WP_049685684.1 bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase -
TKV_RS09210 1834070..1834486 - 417 WP_049685685.1 holo-ACP synthase -
TKV_RS09215 1834585..1834950 - 366 WP_049685686.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1829652..1843149 13497


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12933.02 Da        Isoelectric Point: 6.4714

>T48537 WP_003867472.1 NZ_CP009170:c1830002-1829652 [Thermoanaerobacter kivui]
MVIKRGDIFYADLSPVIGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPTHVEINGAEYGLNKDSVVLLEQI
RTIDKKRLREKIGHFDQEMMEKVNEALQISLGLIDF

Download         Length: 351 bp

>T48537 NZ_CP009170:c1830002-1829652 [Thermoanaerobacter kivui]
ATGGTGATAAAGAGAGGAGATATCTTTTATGCCGATTTAAGCCCTGTAATAGGCTCTGAACAGGGTGGAATTCGGCCTGT
ACTTATAATTCAAAATGATATTGGTAATAAATATAGTCCAACAGTAATAGTAGCGGCAATTACATCTCAGATCAACAAAG
CCAAATTGCCCACTCATGTTGAAATAAATGGGGCAGAATATGGACTTAACAAAGATTCTGTAGTATTGTTAGAACAAATC
AGGACTATCGACAAAAAACGTTTAAGAGAAAAAATTGGCCATTTTGACCAGGAAATGATGGAGAAAGTCAATGAAGCTTT
GCAAATAAGTTTAGGGTTGATTGATTTTTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10547.32 Da        Isoelectric Point: 6.3662

>AT48537 WP_003870023.1 NZ_CP009170:c1830280-1830005 [Thermoanaerobacter kivui]
VGETKRILVSLPQSLLEEVDVLAAMENRNRSEFIREAMKLYIRERKKVQIRESMKKGYLEMAAINSELAEMGLTAENECF
TGYEMKLKKCD

Download         Length: 276 bp

>AT48537 NZ_CP009170:c1830280-1830005 [Thermoanaerobacter kivui]
GTGGGCGAAACAAAGCGAATACTTGTAAGCTTACCTCAGAGTTTATTAGAAGAGGTCGATGTTCTTGCCGCTATGGAAAA
CAGAAATCGCAGTGAATTTATAAGAGAAGCGATGAAATTATATATACGCGAGAGGAAAAAAGTTCAAATACGCGAGAGCA
TGAAAAAAGGATATCTCGAGATGGCAGCAATTAATAGTGAACTTGCGGAAATGGGCCTAACCGCAGAAAATGAATGTTTT
ACAGGATATGAAATGAAATTGAAAAAGTGTGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B3BRL3

Antitoxin

Source ID Structure
AlphaFold DB M8DD10

References