Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 62033..62634 Replicon plasmid p1303_95
Accession NZ_CP009168
Organism Escherichia coli 1303

Toxin (Protein)


Gene name doc Uniprot ID U9Z1Q0
Locus tag EC1303_RS25205 Protein ID WP_001216047.1
Coordinates 62033..62413 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag EC1303_RS25210 Protein ID WP_001190712.1
Coordinates 62413..62634 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC1303_RS25180 57473..58957 - 1485 WP_000124159.1 hypothetical protein -
EC1303_RS25185 58957..60150 - 1194 WP_000219604.1 terminase -
EC1303_RS25190 60237..60689 - 453 WP_001312282.1 late promoter-activating protein (Gp10) -
EC1303_RS25195 60778..61821 - 1044 WP_023351456.1 DUF968 domain-containing protein -
EC1303_RS25200 61849..62028 - 180 WP_000113019.1 hypothetical protein -
EC1303_RS25205 62033..62413 - 381 WP_001216047.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EC1303_RS25210 62413..62634 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EC1303_RS25215 62707..63096 - 390 WP_000506726.1 DNA repair protein -
EC1303_RS25220 63220..63471 - 252 WP_001283837.1 DNA polymerase III subunit theta -
EC1303_RS27895 63645..63854 + 210 WP_001344848.1 hypothetical protein -
EC1303_RS25230 63839..64123 - 285 WP_001142394.1 hypothetical protein -
EC1303_RS25235 64107..64757 - 651 WP_000057451.1 hypothetical protein -
EC1303_RS25240 64739..65113 - 375 WP_000988657.1 hypothetical protein -
EC1303_RS25245 65120..65413 - 294 WP_000269004.1 hypothetical protein -
EC1303_RS25255 65592..65825 - 234 WP_000517421.1 hypothetical protein -
EC1303_RS25260 65908..66777 - 870 WP_041124254.1 DUF551 domain-containing protein -
EC1303_RS28550 66774..67106 - 333 WP_157794728.1 hypothetical protein -
EC1303_RS29090 67111..67289 - 179 Protein_74 hypothetical protein -
EC1303_RS25270 67291..67479 - 189 WP_000797279.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..94959 94959


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13574.27 Da        Isoelectric Point: 5.1514

>T48536 WP_001216047.1 NZ_CP009168:c62413-62033 [Escherichia coli 1303]
MRHISPEELVALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T48536 NZ_CP009168:c62413-62033 [Escherichia coli 1303]
ATGAGACATATATCACCGGAAGAACTTGTTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT48536 WP_001190712.1 NZ_CP009168:c62634-62413 [Escherichia coli 1303]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT48536 NZ_CP009168:c62634-62413 [Escherichia coli 1303]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9Z1Q0

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References