Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 49267..49792 Replicon plasmid p1303_109
Accession NZ_CP009167
Organism Escherichia coli 1303

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag EC1303_RS24545 Protein ID WP_001159871.1
Coordinates 49487..49792 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag EC1303_RS24540 Protein ID WP_000813639.1
Coordinates 49267..49485 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC1303_RS24520 44546..44650 - 105 WP_001443026.1 IS3 family transposase -
EC1303_RS24525 44678..45514 - 837 WP_041124238.1 helix-turn-helix domain-containing protein -
EC1303_RS28500 46197..47162 - 966 Protein_34 IS3 family transposase -
EC1303_RS24540 49267..49485 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EC1303_RS24545 49487..49792 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
EC1303_RS24550 49821..50600 + 780 WP_050544099.1 site-specific integrase -
EC1303_RS24555 51321..52076 + 756 WP_000852146.1 replication initiation protein RepE -
EC1303_RS24560 52573..53739 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -
EC1303_RS24565 53739..54710 + 972 WP_000817031.1 ParB/RepB/Spo0J family plasmid partition protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..108501 108501


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T48530 WP_001159871.1 NZ_CP009167:49487-49792 [Escherichia coli 1303]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T48530 NZ_CP009167:49487-49792 [Escherichia coli 1303]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCTGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT48530 WP_000813639.1 NZ_CP009167:49267-49485 [Escherichia coli 1303]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT48530 NZ_CP009167:49267-49485 [Escherichia coli 1303]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References