Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4633923..4634420 Replicon chromosome
Accession NZ_CP009166
Organism Escherichia coli 1303

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EC1303_RS22650 Protein ID WP_001173343.1
Coordinates 4634247..4634420 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EC1303_RS22645 Protein ID WP_000398619.1
Coordinates 4633923..4634219 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC1303_RS22620 4630654..4631373 - 720 WP_000611288.1 two-component system response regulator DcuR -
EC1303_RS22625 4631370..4633001 - 1632 WP_021571028.1 sensor histidine kinase -
EC1303_RS22630 4633182..4633412 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
EC1303_RS22635 4633424..4633696 + 273 WP_000405651.1 N-acetyltransferase -
EC1303_RS22645 4633923..4634219 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EC1303_RS22650 4634247..4634420 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EC1303_RS22655 4634539..4636056 - 1518 WP_001295074.1 lysine--tRNA ligase -
EC1303_RS22660 4636293..4637750 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T48529 WP_001173343.1 NZ_CP009166:4634247-4634420 [Escherichia coli 1303]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T48529 NZ_CP009166:4634247-4634420 [Escherichia coli 1303]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT48529 WP_000398619.1 NZ_CP009166:4633923-4634219 [Escherichia coli 1303]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT48529 NZ_CP009166:4633923-4634219 [Escherichia coli 1303]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References