Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3211951..3212605 Replicon chromosome
Accession NZ_CP009166
Organism Escherichia coli 1303

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag EC1303_RS15730 Protein ID WP_000244777.1
Coordinates 3211951..3212358 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EC1303_RS15735 Protein ID WP_000354046.1
Coordinates 3212339..3212605 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC1303_RS15710 3207908..3209641 - 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -
EC1303_RS15715 3209647..3210357 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EC1303_RS15720 3210382..3211278 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EC1303_RS15725 3211390..3211911 + 522 WP_001055874.1 flavodoxin FldB -
EC1303_RS15730 3211951..3212358 - 408 WP_000244777.1 toxin CptA Toxin
EC1303_RS15735 3212339..3212605 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EC1303_RS15740 3212848..3213828 + 981 WP_001514522.1 tRNA-modifying protein YgfZ -
EC1303_RS15745 3214024..3214683 - 660 WP_000250274.1 hemolysin III family protein -
EC1303_RS15750 3214847..3215158 - 312 WP_001514524.1 N(4)-acetylcytidine aminohydrolase -
EC1303_RS15755 3215203..3216636 + 1434 WP_001514525.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T48524 WP_000244777.1 NZ_CP009166:c3212358-3211951 [Escherichia coli 1303]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T48524 NZ_CP009166:c3212358-3211951 [Escherichia coli 1303]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT48524 WP_000354046.1 NZ_CP009166:c3212605-3212339 [Escherichia coli 1303]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT48524 NZ_CP009166:c3212605-3212339 [Escherichia coli 1303]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References