Detailed information of TA system TA048513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1806724..1807250
• Replicon: chromosome
• Accession: NZ_CP009166
• Organism: Escherichia coli 1303

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: EC1303_RS08795
• Protein ID: WP_000323025.1
• Coordinates: 1806724..1807011 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: EC1303_RS08800
• Protein ID: WP_000534858.1
• Coordinates: 1807011..1807250 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC1303_RS08755	1801748..1801963	-	216	WP_000839590.1	phage lysis protein EssD	-
EC1303_RS08760	1802717..1802932	-	216	WP_000066484.1	cold shock-like protein CspB	-
EC1303_RS08765	1803233..1803445	+	213	WP_000087756.1	cold shock-like protein CspF	-
EC1303_RS28810	1803500..1803589	+	90	WP_120795389.1	hypothetical protein	-
EC1303_RS08770	1803867..1804619	-	753	WP_001047135.1	antitermination protein	-
EC1303_RS08775	1804633..1805682	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
EC1303_RS08780	1805684..1805962	-	279	WP_012304870.1	hypothetical protein	-
EC1303_RS08785	1806029..1806280	-	252	WP_000980994.1	hypothetical protein	-
EC1303_RS08790	1806497..1806652	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
EC1303_RS08795	1806724..1807011	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
EC1303_RS08800	1807011..1807250	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
EC1303_RS26475	1807275..1807580	+	306	WP_001326990.1	hypothetical protein	-
EC1303_RS08805	1807783..1808115	+	333	WP_001301033.1	protein FlxA	-
EC1303_RS08815	1808573..1809735	+	1163	WP_089566769.1	IS3-like element IS3 family transposase	-
EC1303_RS08820	1809793..1811106	-	1314	WP_000589005.1	ISNCY family transposase	-
EC1303_RS29445	1811875..1812162	-	288	Protein_1685	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1787319..1818972	31653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T48513 WP_000323025.1 NZ_CP009166:c1807011-1806724 [Escherichia coli 1303]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T48513 NZ_CP009166:c1807011-1806724 [Escherichia coli 1303]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT48513 WP_000534858.1 NZ_CP009166:c1807250-1807011 [Escherichia coli 1303]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT48513 NZ_CP009166:c1807250-1807011 [Escherichia coli 1303]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8