Detailed information of TA system TA048509

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 979941..980776
• Replicon: chromosome
• Accession: NZ_CP009166
• Organism: Escherichia coli 1303

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0E1LX62
• Locus tag: EC1303_RS04640
• Protein ID: WP_000854745.1
• Coordinates: 980399..980776 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0E1LVG0
• Locus tag: EC1303_RS04635
• Protein ID: WP_001285484.1
• Coordinates: 979941..980309 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC1303_RS04600	976189..976644	+	456	WP_000581509.1	hypothetical protein	-
EC1303_RS04605	976723..976956	+	234	WP_001119730.1	DUF905 family protein	-
EC1303_RS04610	977056..977874	+	819	WP_001234691.1	DUF945 domain-containing protein	-
EC1303_RS04615	977965..978450	+	486	WP_000213715.1	antirestriction protein	-
EC1303_RS04620	978466..978942	+	477	WP_001186768.1	RadC family protein	-
EC1303_RS04625	979011..979232	+	222	WP_000692303.1	DUF987 domain-containing protein	-
EC1303_RS04630	979247..979891	+	645	WP_000086750.1	hypothetical protein	-
EC1303_RS04635	979941..980309	+	369	WP_001285484.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EC1303_RS04640	980399..980776	+	378	WP_000854745.1	TA system toxin CbtA family protein	Toxin
EC1303_RS04645	980773..981261	+	489	WP_041124065.1	hypothetical protein	-
EC1303_RS04650	981273..981470	+	198	WP_000839242.1	DUF957 domain-containing protein	-
EC1303_RS04655	981555..982397	+	843	WP_001280423.1	DUF4942 domain-containing protein	-
EC1303_RS04665	982888..983106	-	219	WP_001040187.1	translation initiation factor IF-1	-
EC1303_RS04670	983391..984095	-	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14071.99 Da
• Isoelectric Point: 7.9308

>T48509 WP_000854745.1 NZ_CP009166:980399-980776 [Escherichia coli 1303]
MKTLPDTHVREASRSPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T48509 NZ_CP009166:980399-980776 [Escherichia coli 1303]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCAGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCTC
CCGACTACTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13647.28 Da
• Isoelectric Point: 5.9573

>AT48509 WP_001285484.1 NZ_CP009166:979941-980309 [Escherichia coli 1303]
VSDTLHETNYPDDNNDRPWWGLPSTVTPCFGTRLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLGLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTSETKK

Nucleotide

• Length: 369 bp

>AT48509 NZ_CP009166:979941-980309 [Escherichia coli 1303]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAATAACGACCGCCCCTGGTGGGGACTACCCAGCACCGTGAC
GCCCTGTTTCGGGACACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGGACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCTGATACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E1LX62

Antitoxin

• AlphaFold DB: A0A0E1LVG0