Detailed information of TA system TA048500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 889932..890545
• Replicon: chromosome
• Accession: NZ_CP009163
• Organism: Burkholderia pseudomallei K42

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: X993_RS21480
• Protein ID: WP_050019426.1
• Coordinates: 889932..890240 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: X993_RS21485
• Protein ID: WP_044490809.1
• Coordinates: 890237..890545 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
X993_RS21440	885970..886191	+	222	WP_044490808.1	hypothetical protein	-
X993_RS36210	886184..886462	+	279	WP_080296981.1	hypothetical protein	-
X993_RS21445	886485..886784	+	300	WP_044490033.1	hypothetical protein	-
X993_RS21450	886813..887319	+	507	WP_044490034.1	hypothetical protein	-
X993_RS21455	887319..888014	+	696	WP_044490035.1	hypothetical protein	-
X993_RS21460	888066..888350	+	285	WP_044490036.1	hypothetical protein	-
X993_RS21465	888347..888649	+	303	WP_044490037.1	hypothetical protein	-
X993_RS21470	888646..888975	+	330	WP_044490038.1	hypothetical protein	-
X993_RS21475	889018..889575	+	558	WP_044490039.1	hypothetical protein	-
X993_RS21480	889932..890240	+	309	WP_050019426.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
X993_RS21485	890237..890545	+	309	WP_044490809.1	putative addiction module antidote protein	Antitoxin
X993_RS21490	890708..891157	-	450	WP_044490810.1	hypothetical protein	-
X993_RS21495	892123..893157	+	1035	WP_004521858.1	LacI family DNA-binding transcriptional regulator	-
X993_RS21500	893214..894275	+	1062	WP_004193494.1	ABC transporter substrate-binding protein	-
X993_RS21505	894370..895194	+	825	WP_004191802.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	869615..890545	20930

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (7-98)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.99 Da
• Isoelectric Point: 7.0117

>T48500 WP_050019426.1 NZ_CP009163:889932-890240 [Burkholderia pseudomallei K42]
MNTINTTDEFSEWLTDLKDQKAKGAIIARIRRASLDNFGDYKDLGDGVSEMKIDVGPGYRVYYAREGRTVYLLISGGTKS
SQDADIRHAKAIWEQFKKEQQP

Nucleotide

• Length: 309 bp

>T48500 NZ_CP009163:889932-890240 [Burkholderia pseudomallei K42]
ATGAACACCATCAACACCACCGACGAGTTCTCAGAATGGCTGACCGATCTCAAGGATCAGAAGGCCAAGGGTGCGATCAT
CGCTCGCATCCGCCGGGCCTCGCTCGACAACTTCGGTGATTACAAAGACCTTGGAGACGGTGTTTCGGAGATGAAAATCG
ATGTGGGCCCCGGGTATCGGGTCTACTACGCTAGGGAGGGACGTACCGTCTATTTACTCATTTCAGGTGGCACCAAATCT
TCGCAGGATGCCGATATCCGGCACGCGAAAGCCATCTGGGAGCAATTCAAGAAGGAGCAGCAGCCATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10896.41 Da
• Isoelectric Point: 5.1409

>AT48500 WP_044490809.1 NZ_CP009163:890237-890545 [Burkholderia pseudomallei K42]
MSTAEKIKTAPFDASNYLDSEEAIAAYINAAIEADDQDLLLLAIADVAKARGMAKVAADAGLGRESLYKALARGANPRFE
TVNKLMHALGVRFEAVPDRKTS

Nucleotide

• Length: 309 bp

>AT48500 NZ_CP009163:890237-890545 [Burkholderia pseudomallei K42]
ATGAGCACGGCTGAGAAGATCAAGACCGCGCCATTCGACGCCTCGAACTATCTCGACAGCGAAGAAGCGATAGCCGCCTA
CATCAATGCGGCTATCGAAGCGGACGATCAAGACCTACTGCTACTCGCCATCGCGGACGTAGCCAAAGCCCGCGGCATGG
CCAAAGTAGCCGCCGATGCCGGCCTGGGCAGGGAGAGCCTGTACAAGGCGCTTGCCCGAGGTGCGAATCCGCGGTTCGAG
ACCGTCAACAAGCTGATGCACGCCCTCGGCGTCCGCTTTGAGGCAGTACCTGACCGCAAGACCTCATAG