Detailed information of TA system TA048499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 876728..877265
• Replicon: chromosome
• Accession: NZ_CP009163
• Organism: Burkholderia pseudomallei K42

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: X993_RS21370
• Protein ID: WP_004549731.1
• Coordinates: 876996..877265 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: X993_RS21365
• Protein ID: WP_004525420.1
• Coordinates: 876728..877012 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
X993_RS21340	871837..873099	-	1263	WP_004537187.1	c-type cytochrome	-
X993_RS21345	873258..874718	-	1461	WP_004547073.1	metallophosphoesterase	-
X993_RS21350	874965..875081	+	117	Protein_682	IS3 family transposase	-
X993_RS36190	875308..875736	-	429	Protein_683	IS110 family transposase	-
X993_RS36195	875699..876082	-	384	Protein_684	DDE-type integrase/transposase/recombinase	-
X993_RS21365	876728..877012	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
X993_RS21370	876996..877265	-	270	WP_004549731.1	BrnT family toxin	Toxin
X993_RS21375	877818..878093	+	276	WP_038726742.1	hypothetical protein	-
X993_RS21390	878370..878591	+	222	WP_029248167.1	hypothetical protein	-
X993_RS40975	878558..878857	+	300	WP_161788725.1	hypothetical protein	-
X993_RS21400	879088..879669	-	582	WP_004542548.1	hypothetical protein	-
X993_RS21405	879925..880074	+	150	WP_004537646.1	bacteriophage protein Gp44	-
X993_RS41455	880071..880844	+	774	Protein_692	prohibitin family protein	-
X993_RS41610	880842..881270	+	429	Protein_693	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	869615..890545	20930

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T48499 WP_004549731.1 NZ_CP009163:c877265-876996 [Burkholderia pseudomallei K42]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T48499 NZ_CP009163:c877265-876996 [Burkholderia pseudomallei K42]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT48499 WP_004525420.1 NZ_CP009163:c877012-876728 [Burkholderia pseudomallei K42]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT48499 NZ_CP009163:c877012-876728 [Burkholderia pseudomallei K42]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: Q2T6G2

Antitoxin

• AlphaFold DB: A0A0F6L9S8