Detailed information of TA system TA048493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 139166..139703
• Replicon: chromosome
• Accession: NZ_CP009160
• Organism: Burkholderia pseudomallei TSV 48

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: X988_RS00555
• Protein ID: WP_009923450.1
• Coordinates: 139434..139703 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: X988_RS00550
• Protein ID: WP_004525420.1
• Coordinates: 139166..139450 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
X988_RS00525	134274..135536	-	1263	WP_038765621.1	c-type cytochrome	-
X988_RS00530	135695..137155	-	1461	WP_038765619.1	metallophosphoesterase	-
X988_RS00535	137402..137518	+	117	Protein_103	IS3 family transposase	-
X988_RS31910	137745..138173	-	429	Protein_104	IS110 family transposase	-
X988_RS00540	138136..138519	-	384	Protein_105	DDE-type integrase/transposase/recombinase	-
X988_RS00550	139166..139450	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
X988_RS00555	139434..139703	-	270	WP_009923450.1	BrnT family toxin	Toxin
X988_RS00560	140274..140549	+	276	WP_004552388.1	bacteriophage protein Gp49	-
X988_RS00565	140559..140690	+	132	WP_009923452.1	bacteriophage protein Gp48	-
X988_RS00575	140826..141047	+	222	WP_038756244.1	hypothetical protein	-
X988_RS40640	141014..141313	+	300	WP_160458000.1	hypothetical protein	-
X988_RS00585	141544..142125	-	582	WP_004542548.1	hypothetical protein	-
X988_RS00590	142381..142530	+	150	WP_004542626.1	bacteriophage protein Gp44	-
X988_RS41285	142527..143300	+	774	Protein_114	prohibitin family protein	-
X988_RS41290	143298..143726	+	429	Protein_115	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	138136..146037	7901

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10156.52 Da
• Isoelectric Point: 7.1633

>T48493 WP_009923450.1 NZ_CP009160:c139703-139434 [Burkholderia pseudomallei TSV 48]
MDITFDPTKNKTNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T48493 NZ_CP009160:c139703-139434 [Burkholderia pseudomallei TSV 48]
ATGGACATCACCTTTGACCCGACCAAGAACAAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT48493 WP_004525420.1 NZ_CP009160:c139450-139166 [Burkholderia pseudomallei TSV 48]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT48493 NZ_CP009160:c139450-139166 [Burkholderia pseudomallei TSV 48]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8