Detailed information of TA system TA048492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-couple_hipB
• Location: 2430101..2430743
• Replicon: chromosome
• Accession: NZ_CP009159
• Organism: Actinobacillus suis ATCC 33415

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0GF68
• Locus tag: ASU1_RS11320
• Protein ID: WP_005621659.1
• Coordinates: 2430101..2430472 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: ASU1_RS11325
• Protein ID: WP_005621656.1
• Coordinates: 2430453..2430743 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASU1_RS11305	2426606..2427460	+	855	WP_015674480.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-
ASU1_RS11310	2427504..2428073	-	570	WP_005608594.1	elongation factor P hydroxylase	-
ASU1_RS11315	2428132..2429883	-	1752	WP_015674481.1	protein-disulfide reductase DsbD	-
ASU1_RS11320	2430101..2430472	+	372	WP_005621659.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASU1_RS11325	2430453..2430743	+	291	WP_005621656.1	helix-turn-helix transcriptional regulator	Antitoxin
ASU1_RS11330	2430811..2431773	-	963	WP_015674482.1	calcium/sodium antiporter	-
ASU1_RS11335	2431846..2432343	+	498	WP_015674483.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
ASU1_RS11340	2432571..2433305	+	735	WP_015674484.1	arginine ABC transporter ATP-binding protein ArtP	-
ASU1_RS11345	2433325..2434044	+	720	WP_015674485.1	transporter substrate-binding domain-containing protein	-
ASU1_RS11350	2434050..2434721	+	672	WP_015674486.1	arginine ABC transporter permease ArtQ	-
ASU1_RS11355	2434721..2435404	+	684	WP_015674487.1	arginine ABC transporter permease ArtM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (33-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-85)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14938.10 Da
• Isoelectric Point: 9.5717

>T48492 WP_005621659.1 NZ_CP009159:2430101-2430472 [Actinobacillus suis ATCC 33415]
MYEIVFYRDRRGREPVKEFLQEFINEQQDENRERLHKISHHLTILHLHGTRAGESYIKHLEDRIWQLRPISDCLLFAGIV
RGQFVLLHHFAKSSSRLPKRELERAKSRLADLQERIKDEPHWF

Nucleotide

• Length: 372 bp

>T48492 NZ_CP009159:2430101-2430472 [Actinobacillus suis ATCC 33415]
ATGTATGAAATAGTCTTTTATCGAGACCGACGTGGGCGAGAACCCGTCAAGGAATTCCTGCAGGAATTTATTAATGAACA
ACAAGACGAAAATCGAGAGCGTTTGCACAAAATTAGTCATCATTTGACGATTTTACATTTGCACGGCACTCGAGCAGGCG
AAAGTTATATTAAGCATTTAGAAGATCGTATTTGGCAGTTACGTCCAATTAGCGATTGTTTGCTCTTCGCCGGTATTGTA
CGAGGTCAGTTTGTATTGTTGCATCATTTCGCCAAAAGCAGTAGTCGGCTTCCTAAGCGTGAGCTTGAGCGAGCAAAAAG
TCGTTTGGCAGATCTACAGGAGAGGATAAAAGATGAGCCCCATTGGTTCTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10803.58 Da
• Isoelectric Point: 8.7274

>AT48492 WP_005621656.1 NZ_CP009159:2430453-2430743 [Actinobacillus suis ATCC 33415]
MSPIGSSWTSFEREVFNELEMEENHFRVKLILEIIETRQALGISQRKLEKLSGVKQSMIARVEKGSSNPSLGTLLKLLVP
LGKTLQIVPLDDTHSK

Nucleotide

• Length: 291 bp

>AT48492 NZ_CP009159:2430453-2430743 [Actinobacillus suis ATCC 33415]
ATGAGCCCCATTGGTTCTAGTTGGACATCATTTGAACGAGAAGTGTTTAATGAGTTGGAAATGGAAGAAAACCATTTTCG
TGTTAAATTGATTCTTGAAATTATTGAGACCCGACAGGCGTTAGGTATTTCACAACGTAAATTAGAAAAACTAAGTGGCG
TAAAACAATCGATGATAGCCCGGGTTGAAAAGGGAAGCTCCAATCCTTCTCTTGGTACGTTACTAAAATTACTGGTGCCG
CTGGGGAAAACATTACAAATCGTGCCTTTAGACGATACTCATTCTAAATAA

Structures

Toxin

• AlphaFold DB: A0A0A7MI46